Summary
An ion-pair, liquid chromatographic method for the determination of total gossypol in chicken liver has been developed. It involves hydrolysis of bound gossypol in the presence of ascorbic acid, partitioning into chloroform and isocratic separation on Spherisorb ODS2, 10 μm, column with a methanol-water mobile phase containing octane-1-sulfonate. Precision data based on within-day and between-days variation suggested an overall relative standard deviation of 8.1%, while overall recovery was 81.1%±3.
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Botsoglou, N.A., Spais, A.B. Ion-pair, liquid chromatographic analysis of total gossypol in chicken liver. Chromatographia 33, 174–176 (1992). https://doi.org/10.1007/BF02275901
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DOI: https://doi.org/10.1007/BF02275901