Summary
This study was undertaken to evaluate the HPLC separation and quantification of several low molecular mass aldehydes and ketones, which may be produced during combustion in alcohol-fueled automobiles, by means of their dinitrophenylhydrazone derivatives. Stationary phases (ODS), mobile phase components (acetonitrile or methanol with water) and detection sensitivity at 254 and 365 nm were evaluated. Separation of eight dinitrophenylhydrazones could be achieved in approximately 20 minutes using a Zorbax ODS or a Supelcosil LC-PAH column with a MeOH:H2O (75∶25 v/v) mobile phase. Complete separations were not obtained with either a Nova-Pack C-18 or an Ultrasphere ODS column. The use of acetonitrile-water mobile phases produces poorer resolution at all compositions tested. Quantification of the compounds by several methods was compared, with the lowest standard deviations being seen with the external than 1.5 pmol for each of the 2,4-dinitrophenylhydrazones in the test solution. The method is being applied to the analysis of aldehydes and ketones in the exhaust of automobile engines fueled by ethanolgasoline mixtures.
Similar content being viewed by others
References
D. Grosjean, A. H. Miguel, T. M. Tavares, Atmos. Environ.24B, 101 (1990).
M. Warner, Anal. Chem.62, 499A (1990).
N. I. Sax, “Dangerous Properties of Industrial Materials” 6th ed. Van Nostrand-Rheinhold, New York, 1986.
Associação Brasileira de Normas Técnicas (ABNT), project 5:11.03-018 (1986).
R. I. Tanner, A. H. Miguel, J. B. Andrade, J. S. Gaffney, G. E. Streit, Environ. Sci. Technol.22, 1026 (1988).
D. Schuetzle, T. E. Jensen, D. Nagy, A. Prostak, A. Hochauser, Anal. Chem.63, 1149A (1991).
C. Cye, M. Oehme, J. High Resol. Chromatogr.15, 5 (1992).
D. F. Smith, T. E. Kleindienst, E. E. Hudgens, J. Chromatogr.483, 431 (1989).
R. L. Shriner, R. C. Fuson, D. Y. Curtin, T. C. Morrill, “The Systematic Identification of Organic Compounds”, 6th ed. John Wiley and Sons, New York, 1980.
R. J. Kieber, K. Mopper, Environ. Sci. Technol.24, 1477 (1990).
L. P. Haack, D. L. LaCourse, T. J. Korniski, Anal. Chem.58, 68 (1986).
F. Lipari, S. J. Swarin, J. Chromatogr.242, 297 (1982).
A. Grömping, K. Cammann, Fresenius Z. Anal. Chem.335, 796 (1989).
K. L. Olsen, S. J. Swarin, J. Chromatogr.333, 337 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Coutrim, M.X., Nakamura, L.A. & Collins, C.H. Quantification of 2,4-dinitrophenyl-hydrazones of low molecular mass aldehydes and ketones using HPLC. Chromatographia 37, 185–190 (1993). https://doi.org/10.1007/BF02275859
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02275859