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Chromatographia

, Volume 37, Issue 3–4, pp 168–172 | Cite as

The preparation of sorbents for the analysis of human antithrombin III by means of high performance affinity chromatography

  • A. L. Dawidowicz
  • T. Rauckyte
  • J. Rogalski
Originals

Summary

Antithrombin III (AT III) is an anticoagulant present in blood. It is responsible among other things for blood coagulation and the wrong amount can lead to various health problems. The level of AT III can be taken as an indicator of many pathological states. Due to the very complex composition of blood, high performance affinity chromatography seems to be one of the best methods for the quantitative determination of AT III. The present paper deals with the preparation and properties of sorbents for AT III analysis. The behaviour of the chromatographic packings obtained by the bonding of heparin (used as a complementary ligand interacting with AT III) to cross-linked polysaccharide layers deposited on controlled porosity glass is discussed.

Key Words

Affinity chromatography Controlled porosity glass Antithrombin III Heparin immobilization Serum analysis 

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References

  1. [1]
    Ch. Demers, J. S. Ginsberg, P. Henderson, F. A. Ofosu, J. J. Weitz, M. A. Blaichman, Thrombosis and Haemostasis67, 542 (1992).Google Scholar
  2. [2]
    Ch. Demers, J. S. Ginsberg, M. Johnston, P. Brill-Edwards, A. Panju, Thrombosis and Haemostasis67, 408 (1992).Google Scholar
  3. [3]
    M. A. Hultin, J. McKay, U. Abildgeard, Thrombosis and Haemostasis59, 468 (1988).Google Scholar
  4. [4]
    K. Okamoto, A. Takaki, S. Takeda, H. Katoh, K. Ohsato, Haemostasis22, 17 (1992).Google Scholar
  5. [5]
    R. H. Kauffman, J. J. Veltkamp, N. H. Van Tilburg, L. A. Vananes, Am. J. Med.65, 607 (1978).Google Scholar
  6. [6]
    A. Aasen, Acta Clin. Scan., supl.,522, 211 (1985).Google Scholar
  7. [7]
    R. F. Wilson, M. D. E. Mammen, M. C. Robson, J. P. Heggers, G. Soullieu, P. A. Depoli, Arch. Surg.,121, 635 (1986).Google Scholar
  8. [8]
    P. W. Howie, C. R. M. Prentice, G. P. McNicol, Brit. J. Haemat.,25, 101 (1973).Google Scholar
  9. [9]
    M. K. Fagerhol, U. Abildgaard, Scand. J. Haemat.,7, 10 (1970).Google Scholar
  10. [10]
    Z. Perez, Thromb. Res.,24, 367 (1981).Google Scholar
  11. [11]
    Diagnostyka laboratoryjna w hematologii (Laboratory diagnostic in haematology). S. Pawelski, Ed., PZWL, Warszawa 1990, p. 240.Google Scholar
  12. [12]
    F. L. Zhou, D. Muller, X. Santarelli, J. Jozefonvicz, J. Chromatogr.476, 195 (1989).Google Scholar
  13. [13]
    F. L. Zhou, D. Muller, J. Jozefonvicz, J. Chromatogr.510, 71 (1990).Google Scholar
  14. [14]
    W. Haller, J. Phys. Chem.42, 686 (1965).Google Scholar
  15. [15]
    A. L. Dawidowicz, Polish J. Chemistry66, 313 (1992).Google Scholar
  16. [16]
    W. H. Scouten, Affinity Chromatography: Bioselective adsorption on inert matrices, Volume 59 in Chemical Analysis: A series of monographs on analytical chemistry and its application P. J. Elving and J. D. Winefordner, Eds., John Wiley & Sons, New York 1981, p. 48.Google Scholar
  17. [17]
    Wspólczesna transfuzjologia (Contemporary transfusionlogy), W. Rudowski and S. Pawelski, Eds., PZWL, Warszawa 1985, p. 46.Google Scholar
  18. [18]
    Advertasing materials about controlled porosity glasses edited by Electro-Nucleonics, Inc., 386 Passaic Ave., Fairfield, N.J. 07006, USA.Google Scholar
  19. [19]
    W. H. Scouten, Affinity Chromatography: Bioselective adsorption on inert matrices. Volume 59 in Chemical Analysis: A series of monographs on analytical chemistry and its application. P. J. Elving and J. D. Winefordner, Eds., John Wiley & Sons, New York 1981, pp. 77 and 211.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1993

Authors and Affiliations

  • A. L. Dawidowicz
    • 1
  • T. Rauckyte
    • 1
  • J. Rogalski
    • 2
  1. 1.Department of Chemical Physics and Physicochemical Separation MethodsMaria Curie Sklodowska UniversityLublinPoland
  2. 2.Department of BiochemistryMaria Curie Sklodowska UniversityLublinPoland

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