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Inertness and stability of a maltose-silica for the separation of proteins by HPLC

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Summary

A stationary phase consisting of maltose on a silica support, designed for the separation of proteins, is described. When maltose is coupled to aminopropylsilica an effective shielding of the silica surface can be obtained. A size exclusion experiment is used to investigate pore structure and the behaviour of proteins. The stability of the surface layer is studied, as influenced by silica pretreatments and cross-linking reactions. Inertness is studied via the denaturation of trypsin in columns of different length at a number of temperatures. An example of a derivatization of the maltose layer is given which demonstrates its use as an interactive separation medium.

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References

  1. R. E. Huisden, J. C. Kraak, H. Poppe, J. Chromatogr.508, 289 (1960).

    Google Scholar 

  2. X. Santarelli, D. Muller, J. Jozefonvicz, J. Chromatogr.,443, 55 (1988).

    Google Scholar 

  3. F. E. Regnier, R. Noel, J. Chromat. Sci.,14, 316 (1976).

    Google Scholar 

  4. A. V. Kiselev, T. D. Khokhlova, Y. S. Nikiin, Chromatographia,18, 69 (1984).

    Google Scholar 

  5. P. Larsson, M. Glad, L. Hansson, M. Mansson, S. Ohlson, K. Mosbach, Adv. Chromatogr.,21, 41 (1983).

    Google Scholar 

  6. K. Nilsson, K. Mosbach, Meth. Enzymol.,104, 56 (1984).

    Google Scholar 

  7. J. E. Hodge, B. T. Hofreiter, Meth. Carbo. Chem.,5, 380 (1965).

    Google Scholar 

  8. W. Kopaciewicz, M. A. Rounds, F. E. Regnier, J. Chromatogr.,318, 157 (1985).

    Google Scholar 

  9. B. Mclean, S. Souza, I. H. Holmes, J. Clin. Microbiol.,12, 314 (1980).

    Google Scholar 

  10. E. Engelhardt, D. Mathes, Chromatographia,14, 325 (1981).

    Google Scholar 

  11. B. Ansprach, H. U. Gierlach, K. K. Unger, J. Chromatogr.,443, 45 (1988).

    Google Scholar 

  12. C. T. Mant, J. M. R. Parker, R. S. Hodges, J. Chromatogr.,397, 99 (1987).

    Google Scholar 

  13. M. Gimpel, K. Unger, Chromatographia,16, 117 (1982).

    Google Scholar 

  14. P. Andrew Tice, István Mazsaroff, Nan T. Lin, F. E. Regnier, J. Chromatogr.,410, 43 (1987).

    Google Scholar 

  15. G. Fóti, M. L. Belvito, E. sz. Kováts, J. Chromatogr.,440, 315 (1988).

    Google Scholar 

  16. K. Jones, Chromatographia,25, 443 (1988).

    Google Scholar 

  17. N. Sagliano, Jr., T. R. Floyd, R. A. Hartwick, J. M. Dibussolo, N. T. Miller, J. Chromatogr.,443, 155 (1988).

    Google Scholar 

  18. J. Köhler, D. B. Chase, R. D. Farlee, A. J. Vega, J. J. Kirkland, J. Chromatogr.,353, 275 (1986).

    Google Scholar 

  19. B. Johansson, L. Åhsberg, J. Chromatogr.,351, 136 (1986).

    Google Scholar 

  20. R. E. Majors, M. J. Hopper, J. Chromat. Sci.,12, 767 (1974).

    Google Scholar 

  21. P. T. Mora, J. W. Wood, J. Am. Chem. Soc.,80, 685 (1958).

    Google Scholar 

  22. K. Nilsson, K. Mosbach, Meth. Enzymol.,104, 56 (1984).

    Google Scholar 

  23. J. L. Glajch, J. J. Kirkland, J. Köhler, J. Chromatogr.,384, 81 (1987).

    Google Scholar 

  24. V. R. Anicetti, B. A. Keyt, W. S. Hancock, Trends Biotechnol.7, 342 (1989).

    Google Scholar 

  25. J. A. Asenjo, J. Hong, Editors, Separation, Recovery, and Purification in Biotechnology, ACS Symp. Series 314, 1986.

  26. J. Pace, Biochem. J.,24, 606 (1930).

    Google Scholar 

  27. E. S. Parente, D. B. Wetlaufer, J. Chromatogr.,314, 337 (1984).

    Google Scholar 

  28. S. Y. M. Lau, A. K. Taneja, R. S. Hodges, J. Chromatogr.,317, 129 (1984).

    Google Scholar 

  29. R. H. Ingraham, S. Y. M. Lau, A. K. Taneja, R. S. Hodges, J. Chromatogr.,327, 77 (1985).

    Google Scholar 

  30. S-L. Wu, K. Benedek, B. Karger, J. Chromatogr.,359, 3 (1986).

    Google Scholar 

  31. I. S. Krull, H. S. Stuting, S. C. Krzysko, J. Chromatogr.,442, 29 (1988).

    Google Scholar 

  32. K. Benedek, J. Chromatogr.,458, 93 (1988).

    Google Scholar 

  33. M. T. Aubel, G. Guiochon, J. Chromatogr.,498, 281 (1990).

    Google Scholar 

  34. R. E. Huisden, J. C. Kraak, H. Poppe, J. Chromatogr., accepted.

  35. P. P. H. Tock, G. Stegeman, R. Peerboom, H. Poppe, J. C. Kraak, K. K. Unger, Chromatographia,24, 617 (1987).

    Google Scholar 

  36. H. H. Lauer, D. McManigill, Anal. Chem.,58, 166 (1986).

    Google Scholar 

  37. G. Guiochon, A. Katti, Chromatographia,24, 165 (1987).

    Google Scholar 

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Authors and Affiliations

  1. Laboratory for Analytical Chemistry, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV, Amsterdam, The Netherlands

    R. E. Huisden, T. Ooms, J. C. Kraak & H. Poppe

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  1. R. E. Huisden
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  2. T. Ooms
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  3. J. C. Kraak
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  4. H. Poppe
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Huisden, R.E., Ooms, T., Kraak, J.C. et al. Inertness and stability of a maltose-silica for the separation of proteins by HPLC. Chromatographia 31, 263–271 (1991). https://doi.org/10.1007/BF02275748

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  • DOI: https://doi.org/10.1007/BF02275748

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