Plant and Soil

, Volume 84, Issue 3, pp 311–321 | Cite as

Delayed inoculation and starter nitrogen for enhancing early growth and nitrogen status ofLespedeza cuneata

  • David A. Bender
  • Ronald D. Morse
  • John L. Neal
  • Dale D. Wolf
Article

Summary

Two growth chamber experiments were conducted to determine the response ofLespedeza cuneata (Dumont) G. Don. (sericea lespedeza) to delayed inoculation and low levels of nitrogen fertilization. Nitrogen was supplied either as NH 4 + or as NO 3 in solution. At 0.5 and 5.0 ppm nitrogen early growth and N2(C2H2) fixation was inhibited by NH 4 + and promoted by NO 3 . Inoculation at seeding did not negatively affect growth prior to the onset of N2(C2H2) fixation. Delayed inoculation until the trifoliate stage thus did not increase growth or N2 fixation during the first 40 days of growth. After 40 days, specific nitrogenase activity was highest for plants inoculated at the first trifoliate stage of growth. In contrast, growth and total shoot nitrogen accumulation were higher in plants inoculated at planting. The experimental results suggest that delaying inoculation is not a useful technique for improving early growth ofL. cuneata for surface mine reclamation.

Key words

Acetylene reduction Minesoil Nitrogen fixation Nitrogen source Reclamation Sericea lespedeza Surface mining 

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References

  1. 1.
    Dart P J 1977 Infection and development of leguminous nodules. pp. 367–472.In A Treatise on Dinitrogen Fixation. Section III. Biology. Ed. R W F Hardy and H S Silver. John Wiley and Sons. New York.Google Scholar
  2. 2.
    Dart P J and Pate J S 1959 Nodulation studies in legumes III. The effect of delaying inoculation on the seedling symbiosis of barrel medic,Medicago tribuloides Desr. Aust. J. Biol. Sci. 12, 427–444.Google Scholar
  3. 3.
    Dart P J and Wildon D C 1970 Nodulation and nitrogen fixation byVigna sinensis andVicia atropurpurea: The influence of concentration, form and site of application of combined nitrogen. Aust. J. Agric. Res. 21, 45–56.Google Scholar
  4. 4.
    Eaglesham A R J, Hassouma S and Seegars R 1983 Fertilizer-N effects of N2 fixation by cowpea and soybean. Agron. J. 75, 61–66.Google Scholar
  5. 5.
    Hardy R W F, Holsten R D, Jackson E J and Burns R C 1968 The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation. Plant Physiol. 43, 1185–1207.Google Scholar
  6. 6.
    Hardy R W F and Havelka U D 1976 Photosynthate as a major factor limiting nitrogen fixation by field-grown legumes with emphasis on soybeans pp. 421–439.In Symbiotic Nitrogen Fixation in Plants. Ed. P S Nutman. Cambridge Univ. Press, Oxford.Google Scholar
  7. 7.
    Hoagland D R and Arnon D I 1950 The water culture method of growing plants without soil. Calif. Agric. Expt. Sta. Cir. 347.Google Scholar
  8. 8.
    Laane C, Krone W, Konings W, Haaker H and Veeger C 1980 Short-term effect of ammonium chloride in nitrogen fixation byAzotobacter vinelandii and by bacteriods ofRhizobium leguminosarum. Eur. J. Biochem. 103, 29–46.Google Scholar
  9. 9.
    Layzell D B, Rainbird R M, Atkins C A and Pate J S 1979 Economy of photosynthate use in nitrogenfixing legume nodules: Observations on two contrasting symbioses. Plant Physiol. 64, 888–891.Google Scholar
  10. 10.
    Small J G and Leonard O A 1969 Translocation of14C-labelled photosynthate in legumes as influenced by nitrate N. Am. J. Bot. 56, 187–194.Google Scholar
  11. 11.
    Maynard D N and Barker A V 1969 Studies on the tolerance of plants to ammonium nutrition. J. Am. Soc. Hort. Sci. 94, 235–239.Google Scholar
  12. 12.
    Munns D N 1968 Nodulation ofMedicago sativa in solution culture II. Compensating effects of nitrate and prior nodulation. Plant and Soil 28, 246–257.Google Scholar
  13. 13.
    Munns D N 1968 Nodulation ofMedicago sativa in solution culture III. Effects of nitrate on root hairs and infection. Plant and Soil 29, 33–46.Google Scholar
  14. 14.
    Pate J S and Dart P J 1961 Nodulation studies in legumes III. The effect of inoculum strain and time of application of ammonium nitrate on symbiotic response. Plant and Soil 15, 329–346.Google Scholar
  15. 15.
    Peterson H C and Chesters G 1964 A reliable total nitrogen determination of plant tissue accumulating nitrate nitrogen. Agron. J. 56, 89–90.Google Scholar
  16. 16.
    Reeder J D and Berg W A 1977 Plant uptake of indigenous fertilizer from a Cretaceous shale and coal mine spoils. Soil Sci. Soc. Am. J. 41, 919–921.Google Scholar
  17. 17.
    Reeder J D and Berg W A 1977 Nitrogen mineralization and nitrification in a Cretaceous shale and coal mine spoils. Soil Sci. Soc. Am. J. 41, 922–926.Google Scholar
  18. 18.
    Shanmugam K T, O'Gara F, Andersen K and Valentine R C 1978 Biological nitrogen fixation. Annu. Rev. Plant Physiol. 29, 263–276.Google Scholar
  19. 19.
    Street H E and Sheat D E G 1958 The adsorption and availability of nitrate and ammonia. pp. 150–166.In Encyclopedia of Plant Physiology, Vol. VIII. Ed. W. Rhuland. Springer-Verlag, Berlin.Google Scholar
  20. 20.
    Sundstrom F J 1978 The influence of nitrogen source and rate on spoil grown vegetables. Master's thesis, Virginia Polytechnic Institute and State University, Blacksburg.Google Scholar
  21. 21.
    Sundstrom F J, Neal J L, Morse R D and Bender D A 1983 The effect of delayed inoculation on nitrogen fixation byPhaseolus vulgaris L. grown in minesoil. Commun. Soil Sci. Plant Anal. 14, 15–27.Google Scholar
  22. 22.
    Trinchant J C and Rigaud J 1982 Nitrate and nitrous oxide as inhibitors of nitrogenase from soybean bacteriods. Appl. Environ. Microbiol. 44, 1385–1388.Google Scholar
  23. 23.
    Wilson H A and Stewart G 1955 Ammonification and nitrification in mine spoil. West Virginia Univ. Expt. Sta. Bull. 379T.Google Scholar
  24. 24.
    Wright D L, Blaser R E and Woodruff J M 1978 Seedling emergence as related to temperature and moisture tension. Agron. J. 70, 709–712.Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1985

Authors and Affiliations

  • David A. Bender
    • 1
  • Ronald D. Morse
    • 1
  • John L. Neal
    • 1
  • Dale D. Wolf
    • 1
  1. 1.Departments of Horticulture, (Microbiology Section) Biology and AgronomyVirginia Polytechnic Institute and State UniversityBlacksburgUSA

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