Chromatographia

, Volume 40, Issue 1–2, pp 58–68 | Cite as

Aspects of retention behaviour of steroids in packed column supercritical fluid chromatography

  • M. Hanson
Originals

Summary

A selection of steroids of different polarities and different polar functional groups was investigated in terms of retention and elution profile. It was found that the apparent polarity of steroids is determined not only by the amount and the nature of polar functional groups but also by their intramolecular interactions, shielding effects and molecular shape due to the conformation of the molecule.

Key Words

Supercritical fluid chromatography Packed columns Steroids Functional polar groups 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1995

Authors and Affiliations

  • M. Hanson
    • 1
  1. 1.Schering AGBerlinGermany

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