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Analysis of lignin degradation products by capillary electrophoresis

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Summary

A method for the quantitative analysis of phenolic lignin degradation products by capillary electrophoresis (CE) with on-column UV detection has been developed. The liquid biomass solutions contain low molecular hemicellulosic sugars and phenolic lignin degradation products with various degrees of polymerization. Special attention has been paid to the monomeric phenolic components of lignin degradation fragments, e.g. derivatives of phenolic acids, aldehydes, and alcohols. Uncoated fused silica capillaries and borate-phosphate buffer systems at moderate pH conditions were used in order to separate the compounds of interest. To provide validation of the method, the same samples were analyzed independently by HPLC using gradient elution on a RP-C18 column. As sugar degradation fragment, furan-2-carboxylic acid was detected.

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Authors and Affiliations

  1. Institute for Analytical Chemistry and Radiochemistry, Leopold-Franzens-University Innsbruck, Innrain 52a, 6020, Innsbruck, Austria

    S. Masselter, A. Zemann & O. Bobleter

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  1. S. Masselter
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  2. A. Zemann
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  3. O. Bobleter
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Masselter, S., Zemann, A. & Bobleter, O. Analysis of lignin degradation products by capillary electrophoresis. Chromatographia 40, 51–57 (1995). https://doi.org/10.1007/BF02274608

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  • DOI: https://doi.org/10.1007/BF02274608

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