Summary
A direct sample injection approach for the determination of free catecholamines in urine has been developed. The chromatographic separation is carried out on a 150×4.6 mm HisepTM shielded hydrophobic phase column, where proteins in biological fluids are not retained. The mobile phase consists of 0.03 M sodium citrate, 0.004–0.007 M sodium dodecylsulfate (SDS), 3% n-propanol and 1 mM EDTA with pH 4.2. Amperometric detector is used, the working electrode of which is set at 0.35 V (vs. Ag−AgCl). Norepinephrine, epinephrine and dopamine have linear response ranges of 8 ppb–10 ppm, 5 ppb–10 ppm and 2 ppb–10 ppm, respectively. The analytical results obtained from eight urine samples are consistent with those in the references.
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Wang, D.J., Qu, Y., Hu, P. et al. Determination of free catecholamines in urine by direct injection onto a shielded hydrophobic phase column. Chromatographia 31, 137–142 (1991). https://doi.org/10.1007/BF02274561
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DOI: https://doi.org/10.1007/BF02274561