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Chromatographia

, Volume 31, Issue 3–4, pp 133–136 | Cite as

A method for the determination of histamine in wine by HPLC with precolumn derivatization with phenylisothiocyanate

  • M. Calull
  • R. M. Marcé
  • J. Fábregas
  • F. Borrull
Originals

Summary

A method for determining histamine in wine by precolumn derivatization with PITC (phenylisothiocyanate) with reversed-phase HPLC and UV detection is reported. Histamine can be determined together with the 24 amino acids within 40 min, or separately in a shorter time (less than 4 min) if a prior solid phase extraction clean-up is used.

Key Words

Column liquid chromatography Histamine in wine Pre-column derivatization Solid phase extraction 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1991

Authors and Affiliations

  • M. Calull
    • 1
  • R. M. Marcé
    • 1
  • J. Fábregas
    • 1
  • F. Borrull
    • 1
  1. 1.Department de QuímicaUniversitat de BarcelonaTarragonaSpain

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