, Volume 30, Issue 3–4, pp 215–219 | Cite as

HPLC analysis of aspartame and saccharin in pharmaceutical and dietary formulations

  • A. M. Di Pietra
  • V. Cavrini
  • D. Bonazzi
  • L. Benfenati


A reversed-phase HPLC method has been developed suitable for a reliable quality control of pharmaceutical and dietary formulations containing the synthetic sweeteners aspartame and saccharin. The proposed method is able to separate acesulfame, aspartame and saccharin, and their impurities such as 5-benzyl-3,6-dioxo-2-piperazineacetic acid (the major degradation product of aspartame) and 4-sulphamoylbenzoic acid,o- andp-toluenesulphonamides (the synthesis impurities of saccharin). A convenient solid-phase extraction (SPE) procedure using C-18 sorbent, was also developed for the determination of potential saccharin impurities.

Key Words

Column liquid chromatography Aspartame and saccharin Pharmaceutical formulations 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1990

Authors and Affiliations

  • A. M. Di Pietra
    • 1
  • V. Cavrini
    • 1
  • D. Bonazzi
    • 1
  • L. Benfenati
    • 2
  1. 1.Department of Pharmaceutical SciencesBolognaItaly
  2. 2.Presidio Multizonale di PrevenzioneBolognaItaly

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