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Concentration effect in hyaluronan analysis by size exclusion chromatography

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Summary

The concentration effect in size-exclusion chromatographic analysis of hyaluronans of various relative molecular masses (RMM) has been studied. A critical concentration has been found that is negatively dependent on the hyaluronan molecular mass; the higher the biopolymer molecular mass, the smaller the injected sample where the concentration effect should be taken into account for accurate evaluation of molecular mass distribution. At higher temperatures, however, the concentration effect diminishes significantly.

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Authors and Affiliations

  1. Research Institute of Rheumatic Diseases, SK-92101, Piešťany, Slovak Republic

    E. Orviský

  2. Institute of Experimental Pharmacology, Slovak Academy of Sciences, SK-84216, Bratislava, Slovak Republic

    L. Šoltés

  3. Faculty of Science, Health & Medical Studies, Research Division, North East Wales Institute, CH5 4BR, Deeside, Clwyd, UK

    S. Al Assaf

Authors
  1. E. Orviský
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  2. L. Šoltés
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  3. S. Al Assaf
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Orviský, E., Šoltés, L. & Al Assaf, S. Concentration effect in hyaluronan analysis by size exclusion chromatography. Chromatographia 39, 366–368 (1994). https://doi.org/10.1007/BF02274527

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  • DOI: https://doi.org/10.1007/BF02274527

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