Summary
A recently reported chromatographic method selection strategy has been validated using fifteen drug formulations selected randomly from the Belgium Compendium, 1992. On the basis of the hydrophobic and the acidic — basic properties of the sample components, reversed-phase was recommended as the first choice mode for all formulations. For two multicomponent formulations the range of analyte polarity dictated the need for gradient elution. The commerical software DryLab G/plus® was used for selection of optimum gradient conditions. The results obtained by both isocratic and gradient chromatography are discussed, as is the usefulness of a tailing suppressor in both modes.
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References
T. Hamoir, D. L. Massart, in “Advances in Chromatography”, P. R. Brown, E. Grushka, eds, University of Rhode Island, Kingston, USA, Marcel Dekker, New York 1993; p. 97.
L. R. Snyder, J. J. Kirkland, “Introduction to Modern Liquid Chromatography”, John Wiley & Sons, New York, 2nd Edition, 1979.
J. W. Dolan, D. C. Lommen, L. R. Snyder, J. Chromatogr.485, 91 (1989).
R. Kaliszan, “Quantitative Structure-Chromatographic Retention Relationship”, John Wiley & Sons, New York, 1987.
R. Kaliszan, Anal. Chem.64, 619 (1992).
T. Hamoir, B. Bourguignon, H. Hindriks, D. L. Massart, J. Chromatogr.633, 43 (1993).
T. Hamoir, W. King, S. Kokot, K. Douglas, D. L. Massart, J. Chromatogr. Sci.31, 393 (1993).
T. Hamoir, D. L. Massart, J. Chromatogr., in press.
T. Hamoir, D. L. Massart, Anal. Chim. Acta, submitted for publication.
B. Bourguignon, T. Hamoir, D. L. Massart, in preparation.
R. F. Rekker, “The Hydrophobic Fragmental Constant —Its Derivation and Application”, Elsevier, Amsterdam, 1977.
R. F. Rekker, H. M. de Kort, Eur. J. Med. Chem. Chim. Ther.14, 479 (1979).
T. Hamoir, D. L. Massart, J. Chromatogr. Sci., accepted for publication.
T. Hamoir, Y. Verlinden, D. L. Massart, J. Chromatogr. Sci.32, 14 (1994).
A. Bartha, J. Stahlberg, poster presented at the 15th International Symposium on Column Liquid Chromatography, Basel, Switzerland, 1991.
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Hamoir, T., Bourguignon, B. & Massart, D.L. Validation of a strategic approach to high-performance liquid chromatographic method selection. Chromatographia 39, 339–345 (1994). https://doi.org/10.1007/BF02274523
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DOI: https://doi.org/10.1007/BF02274523