Summary
The generation of a micro-gradient for micro HPLC capillary columns is described. The system used is a modified and improved version of a split flow gradient elution device. Gradient tests have been performed and have shown good reproducibility under all conditions. The system has been successfully coupled with a particle beam LC-MS interface and the analysis of a mixture of twenty seven basic or neutral pesticides is reported.
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Berloni, A., Cappiello, A., Famiglini, G. et al. Generation of split-flow micro-gradients for capillary HPLC. Chromatographia 39, 279–284 (1994). https://doi.org/10.1007/BF02274514
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DOI: https://doi.org/10.1007/BF02274514