Chromatographia

, Volume 39, Issue 5–6, pp 271–278 | Cite as

Micro enzymatic assay coupled to capillary electrophoresis via liquid junction

  • Å. Emmer
  • J. Roeraade
Originals

Summary

A system for capillary electrophoresis combined with enzymatic assay has been evaluated for the two enzymes glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. Instrumentation included a post-column reactor coupled to the separation capillary by a liquid junction. A technique for generating a substrate solution flow into the reactor by utilizing two high voltage supplies is proposed. This method offers a high degree of freedom in optimizing the separation and enzymatic reaction conditions individually. Possibilities for improving the enzymatic assay sensitivity were also examined.

Key Words

Capillary electrophoresis Micro enzymatic assay Post-column reactor Liquid junction 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • Å. Emmer
    • 1
  • J. Roeraade
    • 1
  1. 1.Royal Institute of TechnologyDepartment of Analytical ChemistryStockholmSweden

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