Summary
An improved gas chromatographic method is reported for the determination of unconjugated and solvolysable dehydroepiandrosterone and androsterone in plasma. Free steroids below a level of 1 μg/100 cm3 are determined as heptafluorobutyrates by electron capture detection following extraction and thin-layer chromatographic purification. Free steroids above 1 μg/100 cm3 and steroid sulphates are measured as acetates using flame ionisation detection following solvolysis, extraction and thin-layer chromatography. Control experiments and representative normal values prove that the method is suitable for the determination of steroids in human, rat and dog plasma.
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Fehér, T., Bodrogi, L., Fehér, K.G. et al. Gas chromatographic determination of free and solvalysable dehydroepiandrosterone and androsterone in blood. Chromatographia 10, 86–91 (1977). https://doi.org/10.1007/BF02274465
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DOI: https://doi.org/10.1007/BF02274465