Skip to main content
SpringerLink
Log in

Presence and ontogeny of intestinal and pancreatic phospholipase A2-like proteins in the Red Sea bream,Pagrus major. An immunocytochemical study

  • Published:
Fish Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

We have studied the location and the ontogeny of the digestive enzyme, phospholipase A2 (PLA2) immunohistochemically in the adult and larvae/juvenile of the red sea breamPagrus major by using an antiserum against theNaja naja venom PLA2. The antiserum reacts with at least one enzyme among the PLA2s purified from the fish hepatopancreas or intestine. Although the reactivities were comparatively low, it labelled zymogen granules of the pancreatic acinar cells and secretory materials of certain epithelial cells in the depths of epithelial crypts in the pyloric caeca of the adult. The immunoreactivities of PLA2s were investigated in the viscera of larvae and juveniles of the 0 to 85th day after hatch. In the larvae of the 13th day, accumulation of PLA2-positive zymogen granules in the pancreatic acinar cells were first recognized by the immunostaining. The intensity of the labelling subsequently became stronger and dramatically increased between the 20th and 30th day. This increase appeared to be one of the physiological changes associated with the transition to a new benthic life as juveniles. Lack of PLA2 in the pancreas before the 13th day may suggest the possibility that larvae utilized exogenous PLA2, inherent in their prey, to digest the phospholipids. On the other hand, no reactivity was found in the intestine until the 85th day.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References cited

  • Baragi, V. and Lovell, R.T. 1986. Digestive enzyme activities in striped bass from first feeding through larval development. Trans. Am. Fish. Soc. 115: 478–484.

    Article  Google Scholar 

  • Bauermeister, A.E.M., Pirie, B.J.S. and Sargent, J.R. 1979. An electron microscopic study of lipid absorption in the pyloric caeca of rainbow trout (Salmo gairdnerii) fed wax ester-rich zooplankton. Cell Tiss. Res. 200: 475–486.

    Article  Google Scholar 

  • Buddington, R.K. and Diamond, J.M. 1986. Aristotle revisited: The function of pyloric caeca in fish. Proc. Nat. Acad. Sci. U.S.A. 83: 8012–8014.

    Google Scholar 

  • Carey, M.C., Small, D.M. and Bliss, C.M. 1983. Lipid digestion and absorption. Ann. Rev. Physiol. 45: 651–677.

    Article  Google Scholar 

  • Cousin, J.C.B., Baudin-Laurencin, F. and Gabaudan, J. 1987. Ontogeny of enzymatic activities in fed and fasting turbot,Scophthalmus maximus L. J. Fish Biol. 30: 15–33.

    Google Scholar 

  • Cowey, C.B. and Sargent, J.R. 1979.In Fish Physiology. Vol. 8, pp. 1–69. Bioenergetics and Growth. Edited by W.S. Hoar, D.J. Randall and J.R. Brett. Academic Press, New York.

    Google Scholar 

  • Dabrowski, K. 1982. Proteolytic enzyme activity decline in starving fish alevins and larvae. Env. Biol. Fish. 7: 73–76.

    Article  Google Scholar 

  • De Haas, G.H., Postema, N.M., Nieuwenhuizen, W. and Van Deenen, L.L.M. 1968. Purification and properties of an anionic zymogen of phospholipase A from porcine pancreas. Biochim. Biophys. Acta 159: 118–129.

    PubMed  Google Scholar 

  • Elsbach, P., Weiss, J., Franson, R.C., Beckerdite-Quagliate, S., Schneider, A. and Harris, L. 1979. Separation and purification of a potent bactericidal/permeability-increasing protein and a closely associated phospholipase A2 from rabbit polymorphonuclear leucocytes. J. Biol. Chem. 254: 11000–11009.

    PubMed  Google Scholar 

  • Fänge, R. and Grove, D. 1979. Digestion.In Fish Physiology. Vol. 8, pp. 161–260. Bioenergetics and Growth. Edited by W.S. Hoar, D.J. Randall and J.R. Brett. Academic Press, New York.

    Google Scholar 

  • Fawcett, D.W. 1986. A Textbook of Histology, 11th ed., W.B. Saunders Co., Philadelphia.

    Google Scholar 

  • Frazer, A.J. 1989. Triacylglycerol content as a condition index for fish, bivalve, and crustacean larvae. Can. J. Fish. Aquat. Sci. 46: 1868–1873.

    Google Scholar 

  • Frazer, A.J., Gamble, J.C. and Sargent, J.R. 1988. Changes in lipid content, lipid class composition and fatty acid composition of developing eggs and unfed larvae of cod (Gadus morhua). Mar. Biol. 99: 307–313.

    Article  Google Scholar 

  • Fukuhara, O. 1985. Functional morphology and behavior of early life stages of red sea bream. Bull. Jap. Soc. Sci. Fish. 51: 731–743.

    Google Scholar 

  • Govoni, J.J., Boehlert, G.W. and Watanabe, Y. 1986. The physiology of digestion in fish larvae. Env. Biol. Fish. 16: 59–77.

    Article  Google Scholar 

  • Hakanson, J.L. 1989. Analysis of lipid components for determining the condition of anchovy larvae,Engraulis mordax. Mar. Biol. 102: 143–151.

    Google Scholar 

  • Hakanson, J.L. 1989. Condition of larval anchovy (Engraulis mordax) in the Southern California Bight, as measured through lipid analysis. Mar. Biol. 102: 153–159.

    Google Scholar 

  • Henderson, R.J. and Tocher, D.R. 1987. The lipid composition and biochemistry of freshwater fish. Prog. Lipid Res. 26: 281–347.

    Article  PubMed  Google Scholar 

  • Hsueh, W., Desai, U., Gonzalez-Crussi, F., Lamb, R. and Chu, A. 1981. Two phospholipase pools for prostaglandin synthesis in macrophages. Nature, Lond. 290: 710–713.

    Google Scholar 

  • Iijima, K., Nakamura, M., Uematsu, K. and Kayama, M. 1990. Partial purification and characterization of phospholipase A2 from the hepatopancreas of red sea bream. Bull. Jap. Soc. Sci. Fish. 56: 1331–1339.

    Google Scholar 

  • Kanazawa, A., Teshima, S., Inamori, S. and Matsubara, H. 1983. Effects of dietary phospholipids on growth of the larval red sea bream and knife jaw. Mem. Fac. Fish. Kagoshima Univ. 32: 109–114.

    Google Scholar 

  • Kanazawa, A., Teshima, S.-T. and Sakamoto, M. 1985. Effects of dietary bonito-egg phospholipids and some phospholipids on growth and survival of the larval ayu,Plecoglossus altivelis. Z. Angew. Ichthyol. 4: 165–170.

    Google Scholar 

  • Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of bacteriophage T4. Nature. Lond. 227: 680–685.

    Article  PubMed  Google Scholar 

  • Pedersen, B.H., Nilssen, E.M. and Hjelmeland, K. 1987. Variations in the content of trypsin and trypsinogen in larval herring (Clupea harengus) digesting copepod nauplii. Mar. Biol. 94: 171–181.

    Article  Google Scholar 

  • Selzman, H.M. and Liebelt, R.A. 1962. Paneth cell granule of mouse intestine. J. Cell Biol. 15: 136–139.

    PubMed  Google Scholar 

  • Senegas-Balas, F., Balas, D., Verger, R., de Caro, A., Figarella, C., Ferrato, F., Lechene, P., Bertrand, C. and Ribet, A. 1984. Immunohistochemical localization of intestinal phospholipase A2 in rat paneth cells. Histochem. 81: 581–584.

    Article  Google Scholar 

  • Tanaka, M. 1969a. Studies on the structure and function of the digestive system in teleost larvae-1. Development of the digestive system during prelarval stage. Jap. J. Ichthyol. 16: 1–9.

    Google Scholar 

  • Tanaka, M. 1969b. Studies on the structure and function of the digestive system in teleost larvae-II. Characteristics of the digestive system in larvae at the stage of first feeding. Jap. J. Ichthyol. 16: 41–49.

    Google Scholar 

  • Tanaka, M. 1971. Studies on the structure and function of the digestive system in teleost larvae-III. Development of the digestive system during postlarval stage. Jap. J. Ichthyol. 18: 164–174.

    Google Scholar 

  • Towbin, H., Staehelin, T. and Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Nat. Acad. Sci. U.S.A. 76: 4350–4354.

    Google Scholar 

  • Verger, B., De Haas, G.H., Sarda, L. and Desnuelle, P. 1969. Purification from porcine pancreas of two molecular species with lipase activity. Biochem. Biophys. Acta 188: 272–282.

    PubMed  Google Scholar 

  • Waite, M. 1987. The Phospholipase. Handbook of Lipid Research Vol. 5. Plenum Press, New York.

    Google Scholar 

  • Weinman, S., Ores-Carton, C., Rainteau, D. and Puszkin, S. 1986. Immunoelectron microscopic localization of calmodulin and phospholipase A2 in spermatozoa. I. J. Histochem. Cytochem. 34: 1171–1179.

    Google Scholar 

  • Wightman, P.D., Humes, J.L., Davies, P. and Bonney, R.J. 1981. Identification and characterization of two phospholipase A2 activities in resident mouse peritoneal macrophages. Biochem. J. 195: 427–433.

    PubMed  Google Scholar 

  • Yone, Y. and Fujii, M. 1975a. Studies on nutrition of red sea bream. XI. Effects of ω3 fatty acid supplement in a corn oil diet on growth rate and feed efficiency. Bull. Jap. Soc. Sci. Fish. 41: 73–77.

    Google Scholar 

  • Yone, Y. and Fujii, M. 1975b. Studies on nutrition of red sea bream. XII. Effects of ω3 fatty acid supplement in a corn oil diet on fatty acid composition of fish. Bull. Jap. Soc. Sci. Fish. 41: 79–86.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Marine Biochemistry, Faculty of Applied Biological Science, Hiroshima University, 724, Higashi-Hiroshima, Japan

    N. Iijima

  2. Laboratory of Fish Physiology, Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Kagamiyama 1-4-4, 724, Hiroshima, Japan

    K. Uematsu, M. Kitano & M. Morita

Authors
  1. K. Uematsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Kitano
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Morita
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. Iijima
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Uematsu, K., Kitano, M., Morita, M. et al. Presence and ontogeny of intestinal and pancreatic phospholipase A2-like proteins in the Red Sea bream,Pagrus major. An immunocytochemical study. Fish Physiol Biochem 9, 427–438 (1992). https://doi.org/10.1007/BF02274224

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02274224

Keywords

Search

Navigation