Abstract
The electrophoretically fastest migrating protein in ammocoete serum, AS protein, was isolated and found to be an acidic glycoprotein with a molecular weight of 140,000 Daltons. Purified AS was injected into rabbits to produce a monospecific antiserum that was used in rocket immunoelectrophoresis to quantitate AS at different periods in the life cycle. AS was found to constitute approximately 70% of total serum protein in the ammocoete, remained at about the same value during transformation, and was reduced to 38% of total serum protein in the juvenile adult. In both male and female upstream migrant animals, AS was less than 0.5% of total serum protein. When juveniles were acclimated to seawater over a 10 week period, it was found that their AS level was reduced by 92% to 3.8% of total serum protein. These results are discussed in relation to changes in other serum proteins at various intervals of the lamprey life cycle.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References cited
Beamish, F.W.H. and Potter, I.C. 1972. Timing of changes in the blood, morphology and behaviour ofPetromyzon marinus during metamorphosis. J. Fish. Res. Bd. Can. 29: 1277–1282.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Bradley, T.M. and Rourke, A.W. 1988. Smoltification in steel-head trout (Salmo gairdneri): developmental aspects of plasma constituents. Fish Physiol. Biochem. 5: 39–47.
Filosa, M.F., Sargent, P.A., Fisher, M.M. and Youson, J.H. 1982. An electrophoretic and immunoelectrophoretic characterization of the serum proteins of the adult lamprey,Petromyzon marinus. Comp. Biochem. Physiol. 72B: 521–530.
Filosa, M.F., Sargent, P.A. and Youson, J.H. 1986. An electrophoretic and immunoelectric study of serum proteins during the life cycle of the lamprey,Petromyzon marinus L. Comp. Biochem. Physiol. 83B: 143–149.
Gitlin, D., Pericelli, A. and Gitlin, D. 1973. The presence of α-fetoprotein in sharks and its synthesis by fetal gastrointestinal tract and liver. Comp. Biochem. Physiol. 46B: 207–215.
Hardisty, M.W. and Potter, I.C. 1971. The general biology of adult lampreys.In The Biology of Lampreys. Vol. 1, pp. 127–206. Edited by M.W. Hardisty and I.C. Potter. Academic Press, London.
Hartman, H.K. and Udenfriend, S. 1969. A method for the immediate visualization proteins in acrylamide gels and its use for the preparation of antibodies to enzymes. Anal. Biochem. 30: 391–394.
Howell, J.H., Litman, G. and Pollara, R. 1970. Unique structural relationship of adult and larval sea lamprey haemoglobin. Fdn. Proc. Fed. Am. Socs. Exp. Biol. 29: 889.
Ito, M.A., Filosa, M.F. and Youson, J.H. 1988. In vitro study of serum protein synthesis in the livers of larvae and adults of the lamprey,Petromyzon marinus L. J. Exp. Zool. 245: 256–263.
Johanning, K.M. and Bradley, T.M. 1989. Plasma protein changes in Atlantic salmon (Salmo salar) during parr-smolt transformation. Comp. Biochem. Physiol. 92: 555–560.
Larsen, L.O. 1980. Physiology of adult lampreys with special regard to natural starvation, reproduction, and death after spawning. Can. J. Fish. Aquat. Sci. 37: 1762–1769.
Laurell, C.B. 1965. Antigen-antibody crossed electrophoresis. Anal. Biochem. 10: 358.
Macey, D.J., Webb, J. and Potter, I.C. 1982. Iron levels and major iron binding proteins in the plasma of ammocoetes and adults of the Southern Hemisphere lamprey,Geotria australis Gray. Comp. Biochem. Physiol. 72A: 307–312.
Makos, B.K. and Youson, J.H. 1987. Serum levels of biliverdin and bilirubin in the sea lamprey,Petromyzon marinus L. before and after biliary atresia. Comp. Biochem. Physiol. 87A: 761–764.
Nagano, H., Hosaka, K. and Shukuya, R. 1976. The fastest-migrating component of lamprey serum in zone electrophoresis. Comp. Biochem. Physiol. 54B: 369–374.
Potter, I.C., Hilliard, R.W. and Bird, D.J. 1982. Stages in metamorphosis.In The Biology of Lampreys. Vol. 4B, pp. 137–164. Edited by M.W. Hardisty and I.C. Potter. Academic Press, London.
Rall, D.P., Schwab, P. and Zubrod, C. 1961. Alterations of the plasma proteins at metamorphosis of the lamprey (Petromyzon marinus dorsatus). Science 133: 279–280.
Runyon, R.P. 1985. Fundamentals of Statistics in Biology, Medicine and Health Science.s PWS Publications, Boston.
Schreiber, G. 1987. Synthesis, processing and secretion of plasma proteins by the liver and other organs and their regulation.In The Plasma Proteins. Vol. 5, pp. 294–363. Edited by F. Putnam. Academic Press, New York.
Studier, F.W. 1973. Analysis of bacteriophage T7 early ribonucleic acids and proteins on slab gels. J. Mol. Biol. 79: 237–248.
Takacs, B. 1979. Electrophoresis of proteins in polyacrylamide slab gels.In Immunological Methods. pp. 81–105. Edited by I. Lefkovits and B. Pernis. Academic Press, New York.
Thomas, M.L.H. and McCrimmon, H.R. 1964. Variability in paper electrophoretic patterns of the serum of landlocked sea lampreyPetromyzon marinus L. J. Fish. Res. Bd. Can. 21: 239–246.
Uthe, J.F. and Tsuyuki, H. 1966. Comparative zone electropherograms of muscle myogens and blood proteins of adult and ammocoete lamprey. J. Fish. Res. Bd. Can. 24: 1269–1273.
Weeke, B. 1973. General remarks on principles, equipment, reagents and procedures. Scand. J. Immunol. 2(suppl. 1): 15–35.
Weirich, R.T., Schwartz, H.L. and Oppenheimer, J.H. 1987. An analysis of the interrelationship of nuclear and plasma triiodothyronine in the sea lamprey, lake trout and rat: evolutionary considerations. Endocrinology 120: 664–676.
Youson, J.H. 1980. Morphology and physiology of lamprey metamorphosis. Can. J. Fish. Aquat. Sci. 37: 1687–1710.
Youson, J.H. 1981a. The liver.In The Biology of Lampreys. Vol. 3, pp. 263–332. Edited by M.W. Hardisty and I.C. Potter. Academic Press, London.
Youson, J.H. 1981b. The kidneys.In The Biology of Lampreys. Vol. 3, pp. 192–261. Edited by M.W. Hardisty and I.C. Potter. Academic Press, London.
Youson, J.H. 1981c. The alimentary canal.In The Biology of Lampreys. Vol. 3, pp. 95–189. Edited by M.W. Hardisty and I.C. Potter. Academic Press, London.
Youson, J.H. and Potter, I.C. 1979. A description of the stages in metamorphosis of the anadromous sea lamprey,Petromyzon marinus L. Can. J. Zool. 57: 1808–1817.
Youson, J.H., Sargent, P.A. and Barrett, A. 1987. Serum iron concentration and other blood parameters during the life cycle of the sea lampreyPetromyzon marinus L. Comp. Biochem. Physiol. 88A: 325–330.
Zacharius, R.M., Zell, T., Morrison, J. and Woodlock, J. 1969. Glycoprotein staining following electrophoresis and polyacryalmide slab gels. Anal. Biochem. 30: 148–152.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Filosa, M.F., Ito, M.A. & Youson, J.H. Quantitative changes in a serum protein, AS, during the life cycle of the lamprey,Petromyzon marinus L.. Fish Physiol Biochem 9, 417–426 (1992). https://doi.org/10.1007/BF02274223
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02274223