Abstract
In June, July, and September the activities of five enzymes involved in the carbohydrate and fatty acid metabolism, namely phosphofructokinase (PFK), lactate dehydrogenase (LDH), citrate synthase (CS), cytochrome oxidase (cyt ox) and 3-hydroxy-acyl-CoA dehydrogenase (HAD), were measured in the heart, liver, red muscle, white muscle, and gill lamellae of crucian carp (Carassius carassius). LDH activity was measured in both reducing (LDHr) and oxidizing (LDHo) directions.
The PFK activity in red and white muscle increased significantly between July and September indicating a preparation to winter anoxia by an increased glycylytic capacity in these organs. The HAD activity of the liver had increased significantly (by more than 50%) by September, also a preparation to winter anoxia as HAD is used in the reversed β-oxidation (chain elongation of fatty acids). The LDHr and cyt ox activities in the heart and white muscle were highest in July. This shows that both the anaerobic and aerobic capacities are elevated in mid-summer when water temperature is high and oxygen concentration of the water could fluctuate greatly. The LDHo and CS activities in gill lamellae were lowest in July. The results show that the metabolism of crucian carp is under seasonal influence and that a preparation to winter hypoxia/anoxia could be detected in September.
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References cited
Bass, A., Brdiezka, D., Eyer, P., Hofer, S. and Pette, D. 1969. Metabolic differentiation of distinct muscle types at the level of enzymatic organisation. Eur. J. Biochem. 10: 198–206.
Blazka, P. 1958. The anaerobic metabolism of fish. Physiol. Zool. 31: 117–128.
Crabtree, B. and Newsholme, E.A. 1972. The activities of phosphorylase, hexokinase, phosphofructokinase, lactate dehydrogenase and the glycerol 3-phosphate dehydrogenases in muscles from vertebrates and invertebrates. Biochem. J. 130: 697–705.
Ekberg, D.R. 1962. Anaerobic and aerobic metabolism in gills of the crucian carp adapted to high and low temperatures. Comp. Biochem. Physiol. 5: 123–128.
Holopainen, I.J. and Hyvarinen, H. 1985. Ecology and physiology of crucian carp (Carassius carassius L.) in small Finnish ponds with anoxic conditions in winter. Verh. Internat. Verein. Limnol. 22: 2566–2570.
Hyvarinen, H., Holopainen, I.J. and Piironen, J. 1985. Anaerobic wintering of crucian carp (Carassius carassius L.) 1. Annual dynamics of glycogen reserves in nature. Comp. Biochem. Physiol. 82A: 797–803.
Johnston, I.A. and Bernard, L.M. 1983. Utilization of the ethanol pathway in carp following exposure to anoxia. J. Exp. Biol. 104: 73–78.
Johnston, I.A. and Lucking, M. 1978. Temperature induced variation in the distribution of different types of muscle fiber in the goldfish (Carassius auratus). J. Comp. Physiol. 124: 111–116.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurements with the Folin phenol reagent. J. Biol. Chem. 193: 265–275.
Nagell, B. and Brittain, J.E. 1977. Winter anoxia — a general feature of ponds in cold temperate regions. Int. Rev. Hydrobiol. 62: 821–824.
Newsholme, E.A., Zammit, V.A. and Crabtree, B. 1978. Carbohydrate fuels for the tissues. The role of glucose and glycogen as fuels for muscle. Biochem Soc. Trans. 6: 512–520
Nilsson, G. 1990. Long-term anoxia in crusian carps: changes in the levels of amino acids and monoamine neurotransmitters in the brain, chatecholamines in chromaffin tissue, and liver glygogen. J. Exp. Biol. (In press).
Piironen, J. and Holopainen, I.J. 1986. A note on seasonality in anoxia tolerance of crucian carp (Carassius carassius L.) in the laboratory. Ann. Zool. Fennici 23: 335–338.
Shaklee, J.B., Christiansen, J.A., Sidell, B.D., Prosser, C.L. and Whitt, G.S. 1977. Molecular aspects of temperature acclimation in fish: contributions of changes in enzyme activities and isozyme patterns to metabolic reorganisation in the green sunfish. J. Exp. Zool. 201: 1–20.
Shonk, L.E. and Boxer, G.E. 1964. Enzyme patterns in human tissues. I. Methods for the determination of glycolytic enzymes. Cancer Res. 24: 709–724.
Shoubridge, E.A. and Hochachka, P.W. 1980. Ethanol: Novel end product of vertebrate anaerobic metabolism. Science 209: 11: 308–309.
Sidell, B.D. 1980. Responses of goldfish (Carassius auratus, L.) muscle to acclimation temperature: alterations in biochemistry and proportions of different fiber types. Physiol. Zool. 53: 98–107.
Smit, H., Berg, R.J. Van Den and Hartog, I. Kijn-Den 1974. Some experiments on thermal acclimation in the goldfish (Carassius auratus L.). Neth. J. Zool. 24: 32–49.
Srere, P.A. 1969. Citrate synthase. Meth. Enzymology 13: 3–11.
Van den Thillart, G. and Kesbeke, F. 1978. Anaerobic production of carbon dioxid and ammonia by goldfish (Carassius auratus L.). Comp. Biochem. Physiol. 59A: 393–400.
Van den Thillart, G. and Waarde, A. Van 1985. Teleosts in hypoxia: aspects of anaerobic metabolism. Mol. Physiol. 8: 393–409.
Waversveld, J. Van, Addink, A.D.F. and Thillart, G. Van Den 1989. The anaerobic energy metabolism of goldfish determined by simultaneous direct and indirect calorimetry during anoxia and hypoxia. J. Comp. Physiol. 159B: 263–268.
Whereat, A.F., Orishima, M.W., Nelson, J. and Phillips, S.J. 1969. The location of different synthetic systems for fatty acids in inner and outer mitochondrial membranes from rabbit heart. J. Biol. Chem. 23: 6498–6506.
Whereat, A.F., Hull, F.E. and Orishimo, M.W. 1967. The role of succinate in the regulation of fatty acid synthesis by heart mitochondria. J. Biol. Chem. 242: 4013–4022.
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Lind, Y. Summertime and early autumn activity of some enzymes in the carbohydrate and fatty acid metabolism of the crucian carp. Fish Physiol Biochem 9, 409–415 (1992). https://doi.org/10.1007/BF02274222
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DOI: https://doi.org/10.1007/BF02274222