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Rapid analysis of biopolymers on modified non-porous polystyrene-divinylbenzene particles

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Summary

The use of short columns packed with alkylated nonporous 2.1 μm polystyrene — divinylbenzene particles has enabled the efficient separation of proteins, oligonucleotides, and DNA fragments in less than 60 seconds. An increase in the flow-rate resulted in only a minor reduction in the resolution of the proteins. Careful temperature control and small gradient dead volume were essential if reproducible quantitative results were to be achieved. Analysis at elevated temperatures not only reduced the viscosity of the eleents and, hence, column back-pressure but also resulted in higher column efficiency. The best resolution of proteins was achieved by performing the separation at 80°C whereas the optimum temperatures for the separation of oligonucleotides and DNA fragments ranged from 40–50°C. High speed analysis was used both to control the purity of oligonucleotides following automated solid-phase synthesis and to evaluate the expression of multidrug resistance genes in patients suffering from chronic lymphatic leukemia.

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Authors and Affiliations

  1. Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens-University, Innrain 52a, 6020, Innsbruck, Austria

    C. G. Huber

  2. Department of Analytical Chemistry, Johannes-Kepler-University, Altenbergerstraße 69, 4040, Linz, Austria

    P. J. Oefner & G. K. Bonn

Authors
  1. C. G. Huber
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  2. P. J. Oefner
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  3. G. K. Bonn
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Huber, C.G., Oefner, P.J. & Bonn, G.K. Rapid analysis of biopolymers on modified non-porous polystyrene-divinylbenzene particles. Chromatographia 37, 653–658 (1993). https://doi.org/10.1007/BF02274118

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  • DOI: https://doi.org/10.1007/BF02274118

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