Summary
This report attempts to link several previously unassociated phenomena to the formation of a thick film of adsorbed mobile phase acting as part of the effective stationary phase in SFC. Plots of log k′ vs density were found to have a different shape than is generally accepted. The plots of highly retained solutes appear linear at both low and high densities with a change in slope at intermediate densities where adsorption is know to be most extensive. Efficiency degrades under identical conditions that produce significant adsorption of the mobile phase. Van Deemter curves change shape consistent with an additional “C” term, suggesting the formation of a thick stationary phase under the same conditions known to result in multi-layer adsorption of carbon dioxide onto stationary phases.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
U. van Wasen, I. Swaid, G. M. Schneider, Angewandte Chemie (International Edition)19, 575 (1980).
U. van Wasen, G. M. Schneider, Chromatographia8, 274 (1975).
C. R. Yonker, R. W. Gale, R. D. Smith, J. Phys. Chem.91, 3333 (1987).
T. L. Chester, D. P. Innis, J. High Resolut. Chromatogr.8, 561 (1985).
T. A. Berger, J. Chromatography478, 311 (1989).
M. Novotny, S. R. Springston, S. V. Olesik, J. Steger, Paper 535, 1985 Pittsburg Conference & Exposition, New Orleans, LA, USA, Feb 25 – Mar 1, 1985.
P. J. Schoenmakers, in “Supercritical Fluid Chromatography” R. M. Smith, Ed., RSC Chromatography Monograph Series, Royal Society of Chemistry, London 1988, Chapter 4.
P. J. Schoenmakers, L. G. M. Uunk, Chromatographia24, 51 (1987).
H.-G. Janssen, H. M. J. Snijder, J. R. Rijks, C. A. Cramers, P. J. Schoenmakers, J. High Resolut. Chromatogr.14, 438 (1991).
P. A. Mourier, M. H. Caude, R. H. Rosset, Chromatographia23 (1), 21 (1987).
D. E. Martire, R. E. Boehm, J. Phys. Chem.91, 2433 (1987).
D. P. Poe, D. E. Matire, J. Chromatogr.517, 3 (1990).
D. P. Poe, J. Chromatogr.625, 299 (1992).
T. A. Berger, J. F. Deye, Chromatographia31, 529 (1991).
L. M. Blumberg, T. A. Berger, J. Chromatogr.596, 1 (1992).
T. A. Berger, M. Blumberg, Chromatographia, accepted for publication.
J. R. Strubinger, H. Song, J. F. Parcher, Anal. Chem.63, 98 (1991).
P. J. Schoenmakers, J. Chromatogr.315, 1 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Berger, T.A. The effect of adsorbed mobile phase components on the retention mechanism, efficiency, and peak distortion in supercritical fluid chromatography. Chromatographia 37, 645–652 (1993). https://doi.org/10.1007/BF02274117
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02274117