Summary
An established method for the analysis of chlortetracycline by liquid chromatography using octylsilylated silica gel as the stationary phase was examined in a multicentre study involving five laboratories and a total of six columns. Three chlortetracycline hydrochloride samples were analysed. The main component and the impurities were determined. An analysis of variance, treating each column as a different laboratory, showed an absence of consistent between-laboratory bias and the presence of a significant laboratory-sample interaction. The repeatability and the reproducibility of the method, expressed as relative standard deviations of the result of the determination of chlortetracycline hydrochloride, were calculated to be 0.9% and 1.1% respectively.
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Smets, K., Roets, E., Miller, J.M. et al. Collaborative study of the analysis of chlortetracycline by liquid chromatography on octylsilyl silica gel. Chromatographia 37, 640–644 (1993). https://doi.org/10.1007/BF02274116
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DOI: https://doi.org/10.1007/BF02274116