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Microsomal immobilized-enzyme-reactor for on-line production of glucuronides in a HPLC column

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Summary

Nonsolubilized rat liver microsomes have been noncovalently immobilized on an immobilized artifical membrane (IAM) HPLC chromatographic support to create a microsomal immobilized enzyme reactor (MIC-IMER). The MIC-IMER has been used for the on-line synthesis and analysis of Phase II metabolism conjugates. The activity of the MIC-IMER was investigated by following thein vitro glucuronidation of two well known UDP-glucuronosyltransferase (UDPGT; EC 2.4.1.17) substrates, 4-nitrophenol (4NP) and 4-methylumbelliferone (4Me7OHC). The substrates underwent conjugation with UDPGA and the rate of conjugation reaction in the MIC-IMER was compared with the same reaction in the test-tube.

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  1. T. Alebić-Kolbah

    Present address: Pliva Research Institute, 41000, Zagreb, Croatia

Authors and Affiliations

  1. Department of Oncology, Pharmacokinetics Division, Montreal General Hospital, McGill University, 1650 Cedar Avenue RM B 7113, H3G 1A4, Montreal, PQ, Canada

    T. Alebić-Kolbah & I. W. Wainer

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  1. T. Alebić-Kolbah
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  2. I. W. Wainer
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Alebić-Kolbah, T., Wainer, I.W. Microsomal immobilized-enzyme-reactor for on-line production of glucuronides in a HPLC column. Chromatographia 37, 608–612 (1993). https://doi.org/10.1007/BF02274110

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  • DOI: https://doi.org/10.1007/BF02274110

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