Chromatographia

, Volume 43, Issue 1–2, pp 76–78 | Cite as

HPLC determination of rhodamine B (C.I. 45170) in cosmetic products

  • L. Gagliardi
  • D. De Orsi
  • G. Cavazzutti
  • G. Multari
  • D. Tonelli
Short Communications

Summary

A method is proposed for the extraction, separation, identification and quantitative measurement of rhodamine B in cosmetics samples The colour is extracted with a solution of orthophosphoric acid in either water or DMF (depending on the type of cosmetic) adsorbed on a polyamide column, and then eluted with water and methanol. Further clean-up of the extract is performed by means of a Bond Elut C-18 cartridge, from which rhodamine B is eluted with methanol-water (8∶2). The final eluate is examined by HPLC. Chromatography is performed on a C-18 column with a gradient elution from 50∶50 to 70∶30 of acetonitrile-water, containing 0.1 M sodium perchlorate, in 15 min. A diodearray detector enables peak purity analysis. The method gives recovery values of approximately 90%, or better, and the reproducibility is within 4%.

Key Words

Column liquid chromatography Rhodamine B in cosmetics 

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References

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1996

Authors and Affiliations

  • L. Gagliardi
    • 1
  • D. De Orsi
    • 1
  • G. Cavazzutti
    • 1
  • G. Multari
    • 1
  • D. Tonelli
    • 2
  1. 1.Laboratorio di Chimica del FarmacoInstituto Superiore di SanitàRomeItaly
  2. 2.Dipartimento di Chimica Fisica ed InorganicaUniversità di BolognaBolognaItaly

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