Antonie van Leeuwenhoek

, Volume 13, Issue 1, pp 55–62 | Cite as

A reaction on the formation of hydrogen peroxide by microorganisms in solid media

  • A. Kneteman


In this paper a special culture medium for the detection of H2O2 formed by bacteria producing acid from glucose and by certain moulds is discussed and the preparation of the same is described. This medium can be used with success instead of the blood benzidine medium and was found to be decidedly more sensitive in so far that it allows to trace very small quantities of H2O2. The mineral manganese peroxide as used in this medium differs in many respects from the precipitate obtained by mixing solutions of manganous sulfate and permanganate as used bySöhngen.

Experiments with moulds have given indications as regards the production of a soluble glucose oxidase.


Hydrogen Glucose Sulfate H2O2 Peroxide 
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© Swets en Zeitlinger 1947

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  • A. Kneteman

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