Summary
Using a rapid chemometric methodology to determine the separation factor, α, at different temperatures, Gibbs Helmholtz parameters (Δ (ΔH), Δ (ΔS), Δ (ΔG)) of two adjacent benodiazepines on a chromatogram were obtained from ln α versus T−1 plots. A temperature dependent reversal of the elution order was studied and the mobile phase composition and column temperature were optimized to obtain the best separation. A flow rate of 0.80 ml min−1 with 52.6% methanol in the methanol-water mixture and a column temperature of 48°C gave the most efficient separation of ten benzodiazepines.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
C. Y. Wu, J. S. Cheng, R. Zeng, Chromatographia35, 33 (1993).
L. Watter, J. Zielinski, D. H. Freeman, Anal. Chem.42, 176 (1970).
Z. Withiewicz, J. Szulc, A. Ziolek, R. Dabrowski, J. Dziaduszek, J. Chromatogr.246, 37 (1982).
Jin Chu-Yong, Ge Haishong, Shao Linxian, Shen Guo Pin, Fenxi Huaxue14, 801 (1986).
M. Jung, G. Schmalzing, V. Schurig, J. Chromatogr.552, 43 (1991).
A. Berthod, W.-Y. Li, D. W. Armstrong, Anal. Chem.64, 873 (1992).
Y. Guillaume, G. Guinchard, J. Liq. Chromatogr.17, 2809 (1994).
Y. Guillaume, C. Guinchard, J. Liq. Chromatogr.16, 3457 (1993).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Guillaume, Y., Guinchard, C. Thermodynamic behavior of mixed benzodiazepines by a new liquid chromatographic method. Chromatographia 40, 193–196 (1995). https://doi.org/10.1007/BF02272170
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02272170