Summary
The effects of acetonitrile and bovine serum albumin (BSA) concentrations on the signal intensity and retention behavior of dansylamino acids have been examined by using γ-cyclodextrin-bonded silica gel as the stationary phase in microcolumn liquid chromatography. Fluorescence intensities of dansylamino acids were enhanced by BSA as a mobile phase additive, e.g., detection limits of dansyl derivatives of L-Ala and L-Phe were improved by a factor of 12–18.
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H. N. Singh, W. L. Hinze, Analyst (London)107, 1073 (1982).
O. Jules, S. Scypinski, L. J. Cline Love, Anal. Chim. Acta169, 355 (1985).
G. Patonay, M. E. Rollie, I. M. Warner, Anal. Chem.57, 569 (1985).
H. Singh, W. L. Hinze, Anal. Lett.15, 221 (1982).
D. W. Armstrong, W. L. Hinze, K. H. Bui, H. N. Singh, Anal. Lett.14, 1659 (1981).
T. Kinoshita, F. Iinuma, A. Tsuji, Biochem. Biophys. Res. Commun.51, 666 (1973).
T. Kinoshita, F. Iinuma, A. Tsuji, Biochem. Chem. Pharm. Bull.22, 2413 (1974).
T. Takeuchi, T. Miwa, Anal. Chim. Acta292, 275 (1994).
T. Takeuchi, T. Miwa, Chromatographia38, 555 (1994).
T. C. I. Wilkinson, D. C. Wilton, Biochem. J.238, 419 (1986).
M. Yamaguchi, K. Wada, J. Ishida, M. Nakamura, Analyst (London)117, 1859 (1992).
J. Ishida, K. Abe, M. Yamaguchi, Proceedings of the 55th Annual Symposium on Analytical Chemistry, Toyama, June, 1994, the Japan Society for Analytical Chemistry, 1994, p. 457.
T. Takeuchi, D. Ishii, J. Chromatogr.213, 25 (1981).
T. Takeuchi, A. Asano, D. Ishii, J. Chromatogr.471, 297 (1989).
M. Hoshino, M. Imamura, K. Ikehara, Y. Hama, J. Phys. Chem.85, 1820 (1981).
T. Takeuchi, T. Miwa, J. Chromatogr., in press.
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Takeuchi, T., Miwa, T. Fluorescence enhancement of dansylamino acids by bovine serum albumin as mobile phase additive in microcolumn liquid chromatography. Chromatographia 40, 159–162 (1995). https://doi.org/10.1007/BF02272165
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DOI: https://doi.org/10.1007/BF02272165