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Fluorescence enhancement of dansylamino acids by bovine serum albumin as mobile phase additive in microcolumn liquid chromatography

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Summary

The effects of acetonitrile and bovine serum albumin (BSA) concentrations on the signal intensity and retention behavior of dansylamino acids have been examined by using γ-cyclodextrin-bonded silica gel as the stationary phase in microcolumn liquid chromatography. Fluorescence intensities of dansylamino acids were enhanced by BSA as a mobile phase additive, e.g., detection limits of dansyl derivatives of L-Ala and L-Phe were improved by a factor of 12–18.

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Takeuchi, T., Miwa, T. Fluorescence enhancement of dansylamino acids by bovine serum albumin as mobile phase additive in microcolumn liquid chromatography. Chromatographia 40, 159–162 (1995). https://doi.org/10.1007/BF02272165

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  • DOI: https://doi.org/10.1007/BF02272165

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