Skip to main content
SpringerLink
Log in

A stability-indicating HPLC method for the determination of benzalkonium chloride in 0.5% tramadol ophthalmic solution

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

A high-performance liquid chromatographic procedure employing ultraviolet detection for the analysis of benzalkonium chloride in 0.5% Tramadol ophthalmic solution is reported. The method requires minimal sample pretreatment and is sensitive, accurate, and reproducible. The peak area versus BAK concentration is linear over the range of 50–150% of its label claim of 0.05 mg/mL. The mean absolute recovery of BAK using the described method is 100.2±1.2%, (mean ±SD, n=10). A stress study with heat, acid, base and UV radiation indicates that the method is stabilityindicating with no interference from drug or degradation products.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. E. E. Codd, R. P. Shank, R. B. Raffa, J. L. Vaught, Soc. Neurosci. Abs.16, 370 (1990).

    Google Scholar 

  2. G. Bernatzky, I. Jurna, Eur. J. Pharmacol.120, 75 (1986).

    Google Scholar 

  3. L. Elrod, Jr., T. G. Golich, J. A. Morley, J. Chromatogr.625, 362 (1992).

    Google Scholar 

  4. R. C. Meyer, J. Pharm. Sci.69, 1148 (1980).

    Google Scholar 

  5. M. R. Euerby, J. Clin. Hosp. Pharm.10, 73 (1985).

    Google Scholar 

  6. G. Ambrus, T. Takahashi, P. A. Marty, J. Pharm. Sci.76, 174 (1987).

    Google Scholar 

  7. A. Gomez-Gomar, M. M. Gonzalez-Aubert, J. Garces-Torrents, J. Costa-Segarra, J. Pharm. Biomed. Anal.8, 871 (1990).

    Google Scholar 

  8. D. F. Marsh, L. T. Takahaski, J. Pharm. Sci.72, 521 (1983).

    Google Scholar 

  9. J. J. Halvax, G. Wiese, J. A. Arp, J. M. P. Vermeer, W. P. Van Bennekom, A. Bault, J. Pharm. Biomed. Anal.8, 243 (1990).

    Google Scholar 

  10. R. B. Miller, C. Chen, C. H. Sherwood, J. Liq. Chromatogr.16, 3801 (1993).

    Google Scholar 

  11. G. Parhizkari, R. B. Miller, C. Chen, J. Liq. Chromatogr. accepted

  12. The United States Pharmacopeia XXII, pp. 1558–1567, U. S. Pharmacopeial Convention, Rockville, MD (1990).

Download references

Author information

Author notes

  1. R. B. Miller

    Present address: Fujisawa USA, Inc., Pharmaceutical Sciences, 2045 N. Cornell Avenue, 60160, Melrose Park, IL, USA

Authors and Affiliations

  1. Pharmaceutical Development Department, IOLAB Corporation (a Johnson & Johnson Company), 500 Iolab Drive, 91711, Claremont, CA, USA

    G. Parhizkari, G. Delker, R. B. Miller & C. Chen

Authors
  1. G. Parhizkari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Delker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. B. Miller
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Parhizkari, G., Delker, G., Miller, R.B. et al. A stability-indicating HPLC method for the determination of benzalkonium chloride in 0.5% tramadol ophthalmic solution. Chromatographia 40, 155–158 (1995). https://doi.org/10.1007/BF02272164

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02272164

Key Words

Search

Navigation