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Isolation and purification of digalactosyldiacylglycerols

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Summary

A convenient method for the isolation of digalactosyldiacylglycerols from plant material has been developed using solid phase extraction. Commerical cartridges were utilized to prepare 100 mg fractions of galactolipids containing more than 97% of digalactosyldiacylglycerol. The purity of the fractions was monitored by an isocratic normal phase analytical HPLC procedure, which was optimized using factorial design. The optimized system was successfully scaled up to a semi-preparative HPLC system with a capacity of about 10 mg/injection which was used to further purify the digalactosyldiacylglycerol fraction.

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Author notes

  1. M. H. J. Bergqvist

    Present address: Karlshamns Lipid Teknik AB, Box 66 86, 113-84, Stockholm, Sweden

Authors and Affiliations

  1. Department of Analytical Chemistry, Stockholm University, Box 66 86, 113 84, Stockholm, Sweden

    M. H. J. Bergqvist

  2. Karlshamns Lipid Teknik AB, Box 66 86, 113-84, Stockholm, Sweden

    B. G. Herslöf

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  1. M. H. J. Bergqvist
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  2. B. G. Herslöf
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Bergqvist, M.H.J., Herslöf, B.G. Isolation and purification of digalactosyldiacylglycerols. Chromatographia 40, 129–133 (1995). https://doi.org/10.1007/BF02272159

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  • DOI: https://doi.org/10.1007/BF02272159

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