Chromatographia

, Volume 12, Issue 1, pp 36–37 | Cite as

Separation of methylated ribonucleosides on cation-exchange thin-layer chromatoplates

  • J. Tomasz
Originals

Summary

Three simple TLC systems based on the commercially available cation-exchange chromatoplates (Fixion 50×8) are described for the separation of different mixtures of naturally occurring methylated ribonucleosides.

Keywords

Methylated Analytical Chemistry Organic Chemistry Ribonucleoside 

List of Abbreviations

TLC

thin-layer chromatography

tRNA

transfer ribonucleic acid

mRNA

messenger ribonucleic acid

A

adenosine

m1A

1-methyladenosine

m6A

6-methyladenosine

m26A

6-dimethyladenosine

i6A

6-isopentenyladenosine

C

cytidine

m3C

3-methylcytidine

m5C

5-methylcytidine

G

guanosine

m7G

7-methylguanosine

I

inosine

m1I

1-methylinosine

U

uridine

m3U

3-methyluridine

ϕ

pseudouridine

X

xanthosine

References

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    J. Tomasz, J. Chromatogr.101, 198 (1974).Google Scholar
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    J. Tomasz, Anal. Biochem.68, 226 (1975).Google Scholar
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    J. Tomas, J. Chromatogr.84, 208 (1973).Google Scholar
  4. [4]
    For pKa values see e.g.R. H. Hall. “The Modified Nucleosides in Nucleic Acids”, Columbia University Press, New York (1971), pp. 83.Google Scholar
  5. [5]
    W. E. Cohn, I. Am. Chem. Soc.72, 1471 (1950).Google Scholar
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    H. Rogg, R. Brambilla, G. Keith, M. Staehelin, Nucleic Acids Res.3, 285 (1976).Google Scholar
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    M. Faust, S. Millward, Anal. Biochem.79, 16 (1977).Google Scholar
  8. [8]
    T. W. Munns, H. F. Sims, J. Chromatogr.111, 403 (1975).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1979

Authors and Affiliations

  • J. Tomasz
    • 1
  1. 1.Institute of Biophysics, Biological Research CenterHungarian Academy of SciencesSzegedHungary

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