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Chromatographia

, Volume 12, Issue 1, pp 36–37 | Cite as

Separation of methylated ribonucleosides on cation-exchange thin-layer chromatoplates

  • J. Tomasz
Originals

Summary

Three simple TLC systems based on the commercially available cation-exchange chromatoplates (Fixion 50×8) are described for the separation of different mixtures of naturally occurring methylated ribonucleosides.

Keywords

Methylated Analytical Chemistry Organic Chemistry Ribonucleoside 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

List of Abbreviations

TLC

thin-layer chromatography

tRNA

transfer ribonucleic acid

mRNA

messenger ribonucleic acid

A

adenosine

m1A

1-methyladenosine

m6A

6-methyladenosine

m26A

6-dimethyladenosine

i6A

6-isopentenyladenosine

C

cytidine

m3C

3-methylcytidine

m5C

5-methylcytidine

G

guanosine

m7G

7-methylguanosine

I

inosine

m1I

1-methylinosine

U

uridine

m3U

3-methyluridine

ϕ

pseudouridine

X

xanthosine

References

  1. [1]
    J. Tomasz, J. Chromatogr.101, 198 (1974).Google Scholar
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    J. Tomasz, Anal. Biochem.68, 226 (1975).Google Scholar
  3. [3]
    J. Tomas, J. Chromatogr.84, 208 (1973).Google Scholar
  4. [4]
    For pKa values see e.g.R. H. Hall. “The Modified Nucleosides in Nucleic Acids”, Columbia University Press, New York (1971), pp. 83.Google Scholar
  5. [5]
    W. E. Cohn, I. Am. Chem. Soc.72, 1471 (1950).Google Scholar
  6. [6]
    H. Rogg, R. Brambilla, G. Keith, M. Staehelin, Nucleic Acids Res.3, 285 (1976).Google Scholar
  7. [7]
    M. Faust, S. Millward, Anal. Biochem.79, 16 (1977).Google Scholar
  8. [8]
    T. W. Munns, H. F. Sims, J. Chromatogr.111, 403 (1975).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1979

Authors and Affiliations

  • J. Tomasz
    • 1
  1. 1.Institute of Biophysics, Biological Research CenterHungarian Academy of SciencesSzegedHungary

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