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Optimizing methylation conditions for gas liquid chromatography assay of lactic and succinic acid in biological samples

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Summary

Aqueous standard solutions of lactic and succinic acids were methylated for assay by gas-liquid chromatography. The efficiency of methylation using boron trifluoridemethanol reagent was compared to methylation using a sulfuric acid-methanol mixture; the ratio of sulfuric acid and methanol was also varied. Reactions were done at 100°C/5 min and at room temperature/20 hours. We found the sulfuric acid-methanol was most efficient for a rapid assay; however, the ratio of sulfuric acid and methanol used is critical in the assay of lactic acid and would be especially important in the clinical laboratory using gas-liquid chromatography for analysis of cerebrospinal fluid from suspect meningitis patients or other body fluids.

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Author notes

  1. I. Brook

    Present address: Clinical Laboratory, Children's Hospital National Medical Center, 20010, Washington, D.C., USA

Authors and Affiliations

  1. Department of Medicine, UCLA School of Medicine, 90024, Los Angeles, California

    K. S. Bricknell, I. Brook & S. M. Finegold

  2. Medical Service, VA Wadsworth Hospital Center, 90073, Los Angeles, California, USA

    K. S. Bricknell, I. Brook & S. M. Finegold

Authors
  1. K. S. Bricknell
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  2. I. Brook
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  3. S. M. Finegold
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Bricknell, K.S., Brook, I. & Finegold, S.M. Optimizing methylation conditions for gas liquid chromatography assay of lactic and succinic acid in biological samples. Chromatographia 12, 22–24 (1979). https://doi.org/10.1007/BF02271594

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  • DOI: https://doi.org/10.1007/BF02271594

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