Chromatographia

, Volume 43, Issue 7–8, pp 361–364 | Cite as

Preparation and separation of metallobacteriochlorophylla by reversed-phase high-performance liquid chromatography

  • H. Inoue
  • Y. Nonomura
  • A. Ashizawa
  • I. Tajima
  • M. Nomura
  • N. Nakamura
  • N. Yoshioka
Originals

Summary

Semi-preparative high-performance liquid chromatography has been used for the preparation of copper(II) bacteriochlorophylla [Cu(II)-BChl-a] and zinc(II) bacteriochlorophylla [Zn(II)-BChl-a]. Both compounds are separated on a reversed-phase Inertsil ODS-2 column using a mobile phase of acetone-methanol (25∶75, v/v). The fractionated metallobacteriochlorophylls (M-Bchl-a) are identified by fast atom bombardment mass spectrometry. The spectroscopic parameters such as the wavelength of absorption maxima and the molar extinction coefficients are determined using pure M-Bchl-a obtained by preparative HPLC. The HPLC method proposed here has been demonstrated to be useful for the purification and determination of components of M-Bchl-a.

Key Words

Column liquid chromatography Bacteriochlorophylla Copper(II) bacteriochlorophylla Zinc(II) bacteriochlorophylla 

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References

  1. [1]
    J. A. S. Cavaleiro, K. M. Smith, Talanta33, 963 (1986).Google Scholar
  2. [2]
    K. M. Smith, G. W. Craig, L. A. Kehres, N. W. Pfennig, J. Chromatogr.281, 209 (1983).Google Scholar
  3. [3]
    R. Bonnett, Chem. Soc. Rev. 19 (1995).Google Scholar
  4. [4]
    H. Ushiyama, M. Nishijima, K. Yasuda, H. Kamimura, S. Tabata, T. Nishima, Shokuhin Eiseigaku Zasshi27, 417 (1986).Google Scholar
  5. [5]
    S. Chernomorsky, J. AOAC Internat.77, 756 (1994).Google Scholar
  6. [6]
    S. Li, H. Inoue, Chromatographia33, 567 (1992).Google Scholar
  7. [7]
    K. Furuya, H. Inoue, T. Shirai, Anal. Sci.3, 353 (1987).Google Scholar
  8. [8]
    K. Furuya, N. Ohki, H. Inoue, T. Shirai, Chromatographia25, 319 (1988).Google Scholar
  9. [9]
    H. Inoue, K. Furuya, K. Watanabe, K. Tanaka, T. Shirai, E. Miyoshi, Anal. Sci.4, 599 (1988).Google Scholar
  10. [10]
    H. Inoue, M. Imai, T. Naemura, K. Furuya, Y. Shizuri, J. Chromatogr.645, 259 (1993).Google Scholar
  11. [11]
    C. Houssier, K. Sauer, J. Amer. Chem. Soc.92, 779 (1970).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1996

Authors and Affiliations

  • H. Inoue
    • 1
  • Y. Nonomura
    • 1
  • A. Ashizawa
    • 1
  • I. Tajima
    • 1
  • M. Nomura
    • 1
  • N. Nakamura
    • 1
  • N. Yoshioka
    • 1
  1. 1.Department of Applied ChemistryKeio UniversityYokohamaJapan

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