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Preparation and separation of metallobacteriochlorophylla by reversed-phase high-performance liquid chromatography

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Summary

Semi-preparative high-performance liquid chromatography has been used for the preparation of copper(II) bacteriochlorophylla [Cu(II)-BChl-a] and zinc(II) bacteriochlorophylla [Zn(II)-BChl-a]. Both compounds are separated on a reversed-phase Inertsil ODS-2 column using a mobile phase of acetone-methanol (25∶75, v/v). The fractionated metallobacteriochlorophylls (M-Bchl-a) are identified by fast atom bombardment mass spectrometry. The spectroscopic parameters such as the wavelength of absorption maxima and the molar extinction coefficients are determined using pure M-Bchl-a obtained by preparative HPLC. The HPLC method proposed here has been demonstrated to be useful for the purification and determination of components of M-Bchl-a.

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Authors and Affiliations

  1. Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, 223, Yokohama, Japan

    H. Inoue, Y. Nonomura, A. Ashizawa, I. Tajima, M. Nomura, N. Nakamura & N. Yoshioka

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  1. H. Inoue
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  2. Y. Nonomura
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  3. A. Ashizawa
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  4. I. Tajima
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  5. M. Nomura
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  6. N. Nakamura
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  7. N. Yoshioka
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Inoue, H., Nonomura, Y., Ashizawa, A. et al. Preparation and separation of metallobacteriochlorophylla by reversed-phase high-performance liquid chromatography. Chromatographia 43, 361–364 (1996). https://doi.org/10.1007/BF02271011

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  • DOI: https://doi.org/10.1007/BF02271011

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