, Volume 43, Issue 5–6, pp 237–241 | Cite as

Band broadening arising from stepwise alterations in mobile phase composition in micellar electrokinetic chromatography

  • U. Bütehorn
  • U. Pyell


Gradient elution in micellar electrokinetic chromatography has been proposed as a technique for extending the elution range and adjusting retention factors for optimum values. It is shown that this approach in practice has not only to be evaluated for adjustment of retention factors but also for the reduced efficiency of the chromatographic system arising from alterations in the separation buffer during the run. Stepwise gradient elution, feasible with commercial instrumentation, results in marked peak broadening attributable to effects of the border zone passing the solute zone.

Key Words

Capillary electrophoresis Micellar electrokinetic chromatography Retention factors Band broadening Stepwise modifier gradient 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1996

Authors and Affiliations

  • U. Bütehorn
    • 1
  • U. Pyell
    • 1
  1. 1.Fachbereich Chemie der Philipps-Universität MarburgMarburgGermany

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