Summary
Gradient elution in micellar electrokinetic chromatography has been proposed as a technique for extending the elution range and adjusting retention factors for optimum values. It is shown that this approach in practice has not only to be evaluated for adjustment of retention factors but also for the reduced efficiency of the chromatographic system arising from alterations in the separation buffer during the run. Stepwise gradient elution, feasible with commercial instrumentation, results in marked peak broadening attributable to effects of the border zone passing the solute zone.
Similar content being viewed by others
References
A.T. Balchunas, M.J. Sepaniak, Anal. Chem.60, 617 (1988).
M.J. Sepaniak, D.F. Swaile, A.C. Powell, J. Chromatogr.480, 185 (1989).
A.C. Powell, M.J. Sepaniak, J. Microcol. Sep.2, 278 (1990).
A.C. Powell, M.J. Sepaniak, Anal. Instrum.21, 25 (1993).
S. Terabe, Y. Ishihama, H. Nishi, T. Fukuyama, K. Otsuka, J. Chromatogr.545, 359 (1991).
U. Pyell, U. Bütehorn, Chromatographia40, 175 (1995).
C. Schwer, E. Kenndler, Anal. Chem.63, 1801 (1991).
S. Terabe, K. Otsuka, T. Ando, Anal. Chem.61, 251 (1989).
A. C. Powell, “Optimization of Micellar Electrokinetic Capillary Chromatography with Solvent-Gradients”, thesis, Univ. Tennessee Knoxville, 1993.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bütehorn, U., Pyell, U. Band broadening arising from stepwise alterations in mobile phase composition in micellar electrokinetic chromatography. Chromatographia 43, 237–241 (1996). https://doi.org/10.1007/BF02270987
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02270987