, Volume 17, Issue 6, pp 341–344 | Cite as

Elution characteristics of some solutes used to measure the column void time on reversed-phase stationary phases in micro-HPLC

  • K. Jinno
  • N. Ozaki
  • T. Sato


The elution characteristics of some solutes commonly used to measure column void time on three reversedphase stationary phases in micro-HPLC are described. The elution characteristics of sodium nitrie, sodium nitrate, deuterium oxide, acetone and N,N-dimethylformamide were determined by using styrene-divinylbenzene copolymer, octylsilica and octadecylsilica columns with aqueous methanol and/or acetonitrile eluents. The results indicate that sodium nitrite and sodium nitrate are suitable solutes in measuring column void time, and that the elution behavior of deuterium oxide is almost similar to that of both sodium salts but it appears as two peaks on a C-18 column. One of the two peaks is confirmed as “vacancy” by the use of the micro-HPLC/IR direct monitoring system.

Key Words

Column void time Reversed-phase HPLC Vacancy peak IR direct monitoring 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1983

Authors and Affiliations

  • K. Jinno
    • 1
  • N. Ozaki
    • 1
  • T. Sato
    • 1
  1. 1.School of Materials ScienceToyohashi University of TechnologyToyohashiJapan

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