, Volume 5, Issue 11, pp 275–277 | Cite as

The separation of glycine and all peptides from diglycine up to heptaglycine by a cation exchange resin

An explanation for the elution order
  • H. A. Billiet
Short Communications


An analytical technique has been developed for the separation and quantitative determination of glycine peptides, from glycine to heptaglycine, with a possible extension to octaglycine and nonaglycine. Using an amino acid analyzer, a cation exchange resin and a citrate buffer of pH 3.35 (gradient elution is not required), the complete separation takes 3 hours 30 minutes. The quantitative precision is about 3%.

The elution order can be explained on the basis of two mechanisms:
  • - the pKa-values determine the positions of glycine up to tetraglycine

  • - tetraglycine up to heptaglycine, with equal pKa-values, follow Andrews' equation, Ve=a−b log M, derived for gel chromatography.


Peptide Chromatography Analytical Chemistry Organic Chemistry Citrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Trennung der Glyzin-Peptide von Diglyzin bis zu Heptaglyzin an einer Kationen-Austauschersäule

Eine Erklärung für die Elutionsfolge

Séparation de la glycine et de tous les peptides de la diglycine à l'heptaglycine sur colonne d'échangeur de cations

Explication de l'ordre d'élution


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Copyright information

© Friedr. Vieweg & Sohn GmbH 1972

Authors and Affiliations

  • H. A. Billiet
    • 1
  1. 1.Laboratorium voor Analytische ScheikundeTechnische HogeschoolDelftThe Netherlands

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