, Volume 5, Issue 11, pp 275–277 | Cite as

The separation of glycine and all peptides from diglycine up to heptaglycine by a cation exchange resin

An explanation for the elution order
  • H. A. Billiet
Short Communications


An analytical technique has been developed for the separation and quantitative determination of glycine peptides, from glycine to heptaglycine, with a possible extension to octaglycine and nonaglycine. Using an amino acid analyzer, a cation exchange resin and a citrate buffer of pH 3.35 (gradient elution is not required), the complete separation takes 3 hours 30 minutes. The quantitative precision is about 3%.

The elution order can be explained on the basis of two mechanisms:
  • - the pKa-values determine the positions of glycine up to tetraglycine

  • - tetraglycine up to heptaglycine, with equal pKa-values, follow Andrews' equation, Ve=a−b log M, derived for gel chromatography.


Peptide Chromatography Analytical Chemistry Organic Chemistry Citrate 

Trennung der Glyzin-Peptide von Diglyzin bis zu Heptaglyzin an einer Kationen-Austauschersäule

Eine Erklärung für die Elutionsfolge

Séparation de la glycine et de tous les peptides de la diglycine à l'heptaglycine sur colonne d'échangeur de cations

Explication de l'ordre d'élution


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Copyright information

© Friedr. Vieweg & Sohn GmbH 1972

Authors and Affiliations

  • H. A. Billiet
    • 1
  1. 1.Laboratorium voor Analytische ScheikundeTechnische HogeschoolDelftThe Netherlands

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