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Quantitative Bestimmung von Abscisinsäure mit Hilfe der Hochdruck-Flüssigkeits-Chromatographie

Quantitative determination of abscisic acid by high pressure liquid chromatography

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Summary

The possibility of quantitative determination of abscisic acid using high pressure liquid chromatography is described by both partition chromatography on Aminex A6 and ion-exchange chromatography on AAX (1% exchanger chemically bound to ZIPAX). The detection of abscisic acid is made by the flow photometer through which high sensitivity is achieved. Consequently, as little as 2 ng of the phytohormone can be determined with an accuracy of ±5%.

Zusammenfassung

Es wird die Möglichkeit der quantitativen Bestimmung von Abscisinsäure mit Hilfe der Hochdruck-Flüssigkeits-Chromatographie sowohl durch Verteilungs-Chromatographie an Aminex A 6 als auch durch Ionenaustausch-Chromatographie an AAX (1% Austauscher chemisch an ZIPAX gebunden) beschrieben. Der Nachweis der Abscisinsäure erfolgt im Durchflußphotometer, wodurch eine hohe Empfindlichkeit erreicht wird, so daß noch 2 ng des Phytohormons bei einer Genauigkeit von ±5% erfaßt werden können.

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Authors and Affiliations

  1. Bundesforschungsanstalt für Rebenzüchtung, Geilweilerhof, D-6741, Siebeldingen, Bundesrepublik Deutschland

    A. Rapp, A. Ziegler, O. Bachmann & H. Düring

Authors
  1. A. Rapp
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  2. A. Ziegler
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  3. O. Bachmann
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  4. H. Düring
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Rapp, A., Ziegler, A., Bachmann, O. et al. Quantitative Bestimmung von Abscisinsäure mit Hilfe der Hochdruck-Flüssigkeits-Chromatographie. Chromatographia 9, 44–47 (1976). https://doi.org/10.1007/BF02270597

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  • DOI: https://doi.org/10.1007/BF02270597

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