Summary
The potential of dimethyl sulphoxide (DMSO) as a mobile phase for chromatography on ready-for-use, thin-layers of silica gel, cellulose, and alumina has been examined with ferro- and ferricyanide anions, applied alone or as a mixture, as systems to be separated. The most satisfactory results, with very compact spots, are observed with silica gel thin-layers. The behaviour on cellulose layers is similar to that on paper, but the chromatographic spots are more compact in the former case, and therefore the separation there is much cleaner and more rapid. On alumina layers both the anions are highly adsorbed at the point of application, and ferrocyanide is rapidly oxidized to ferricyanide in the presence of DMSO. Alumina layers are therefore unsuitable for chromatography in the present system. The differentiative behaviour of DMSO towards the two closely similar anions is explained by considering the semipolar nature of the S−O bond, the molecular and electronic structure of DMSO, and the difference in ionic volume and ionic charge of the two anions.
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Shukla, S.K. Dipolar aprotic solvents in thin-layer chromatography of inorganic substances. Chromatographia 6, 84–88 (1973). https://doi.org/10.1007/BF02270543
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DOI: https://doi.org/10.1007/BF02270543