, Volume 30, Issue 1–2, pp 85–90 | Cite as

Total chromatographic optimization (TOCO) I. Simultaneous optimization of many variables in liquid chromatography

  • Y. Hayashi
  • R. Matsuda
  • A. Nakamura


The following chromatographic variables are totally optimized based on the recently developed information theory of optimization: mobile phase composition, column length, flow rate, wavelength, and the amount of internal standard. The optimal internal standard is selected from among six candidates. Two types of optimal conditions (Φ- and ϑ-optimals) are proposed: the Φ-optimal is defined as the most precise analysis (the maximal Φ) while the ϑ-optimal is the most efficient (rapid) analysis (the maximal ϑ). The observation times for the determination of an antipyretics mixture (three components) in liquid chromatography are ca. 50 s for the ϑ-optimal and ca. 8 min for the Φ-optimal. The reliability of the Φ- and ϑ-optimals is verified by experiments.

Key Words

Column liquid chromatography Optimization of variables Information theory Kalman filter 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1990

Authors and Affiliations

  • Y. Hayashi
    • 1
  • R. Matsuda
    • 1
  • A. Nakamura
    • 1
  1. 1.National Institute of Hygienic SciencesTokyoJapan

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