Chromatographia

, Volume 38, Issue 7–8, pp 421–430 | Cite as

A photometric flow measurements method for characterisation of HPLC pumps

  • K. Shoikhet
  • H. Engelhardt
Originals

Summary

The measurement of flow constancy and pulsation amplitudes of HPLC pump based on the photoconversion of malachite green leucocyanide is described. The irradiation time and hence the degree of conversion of the leucocyanide is correlated to flow fluctuations of piston driven HPLC pumps. It is possible to measure the amplitude of pulsations and determine the constancy of the flow rate. Most of the pumps show a flow stability better than ±1% measured as relative standard deviation of the flow rate under HPLC conditions (pressure drop 100 bar at 1 ml/min flow rate). The most expensive pump of those tested showed less than ±0.5% flow instability, however, this result was achieved by installation of a large-volume pulsation damper. The method described also allows flow rate measurements under FIA conditions where there is little or no pressure drop at the pump outlet.

Key Words

Column liquid chromatography Pumps Flow control 

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References

  1. [1]
    K. W. Bonfig, U. Kuipers, Brennst.-Waerme-Kraft41(10), M2-M8 (1989).Google Scholar
  2. [2]
    G. G. Shamovski, Unpublished results.Google Scholar
  3. [3]
    I. Halász, P. Vogtel, J. Chromatogr.142, 241 (1977).Google Scholar
  4. [4]
    H. S. Booth, Ed. “Inorganic Syntheses”, Vol. 1, McGraw-Hill Book Co., New York, N. Y., 1939, p. 37.Google Scholar
  5. [5]
    J. G. Calvert, J. N. Pits, “Photochemistry”, John Wiley & Sons, New York, London, Sidney, 1967, p. 784.Google Scholar
  6. [6]
    J. G. Calvert, H. J. L. Rechen, J. Amer. Chem. Soc.74, 2101 (1952).Google Scholar
  7. [7]
    V. Balzani, V. Carassiti, “Photochemistry of Coordination Compounds”, Academic Press, London & New York, 1970, pp. 169, 221.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • K. Shoikhet
    • 1
  • H. Engelhardt
    • 1
  1. 1.Angewandte Physikalische ChemieUniversität des SaarlandesSaarbrückenGermany

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