Summary
The principles of previously known methods for introducing anchoring functions into a polystyrene matrix for immobilization of ligands are summarized and two recently developed methods are described. Functionalized and reactive radio-derivatized polystyrene can be obtained by exposing the polymer to high energy (1–10 Mrads) irradiation in the presence of small molecules. The surface-immobilized ligands obtained by radio-derivatization are recognizable by ligand-binding antibodies. Also, carboxyl-containing compounds can be covalently attached to highly crosslinked micropellicular polystyrene matrix by carbodiimide. The sorbent prepared by this method can be used for the purification of monoclonal antibodies from hybridoma cell supernatants.
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Varga, J.M., Wongyai, S., Fritsch, P. et al. Novel methods of introducing anchoring functions into a polystyrene matrix for the immobilization of ligands for high-performance affinity chromatography. Chromatographia 30, 527–532 (1990). https://doi.org/10.1007/BF02269799
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DOI: https://doi.org/10.1007/BF02269799