Summary
The determination of catecholamines (CAs) using micellar liquid chromatography with thermal lens spectrophotometric detection has been studied. CAs are oxidized with hexacyanoferrate(III) to aminochromes which are separated with a mobile phase of 0.05 M sodium dodecyl sulphate, 7% propanol and 0.03 M citrate buffer, pH 4.8, on a partially endcapped C18 column. The aminochrome-micelles and aminochrome-stationary phase association constants are evaluated. Using the 488 nm line of an Ar+ laser with 250 mW pump power the limits of detection are about 4 ng mL−1. The technique is applied to the determination of unconjugated CAs in urine using isoproterenol as internal standard.
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Mallols, J.M.S., Camañas, R.M.V. & Ramis-Ramos, G. Determination of catecholamines as aminochromes by micellar liquid chromatography with thermal lens spectrophotometric detection. Chromatographia 38, 365–372 (1994). https://doi.org/10.1007/BF02269782
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DOI: https://doi.org/10.1007/BF02269782