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Preparation and comparison of RP-HPLC of crosslinked ribosomal protein pairs by chromatography on PLRP- and vydac-columns

  • Würzburg Chromatography Colloquium
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Summary

50S subunits ofBacillus stearothermophilus ribosomes were isolated and crosslinked with the homobifunctional reagent diepoxybutane. Two protein-protein crosslinks consisting of the proteins L23-L29 and L3-L19 could be purified on a preparative scale using conventional column chromatography followed by a combination of different HPLC techniques. This procedure allows the isolation of the crosslinks in amounts high enough for the determination of the crosslinked amino acids.

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Abbreviations

DEB:

dieooxybutane

HPLC:

High Performance Liquid Chromatography

B. stearothermophilus, Bststearothermophilus :

Bacillus

PLRP:

Polystyrene/Polyvinylbenzene Reversed Phase

2D-Page:

Two-dimensional Polyacrylamide Gel Electrophoresis

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Authors and Affiliations

  1. Abt. Wittmann, Max-Planck-Institut für Molekulare Genetik, Ihnestr. 63-73, D-1000, Berlin 33

    S. Herwig

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Herwig, S. Preparation and comparison of RP-HPLC of crosslinked ribosomal protein pairs by chromatography on PLRP- and vydac-columns. Chromatographia 30, 696–702 (1990). https://doi.org/10.1007/BF02269746

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  • DOI: https://doi.org/10.1007/BF02269746

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