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Sequence analysis of phosphotyrosine-containing peptides. Determination of PTH-phosphotyrosine by capillary electrophoresis

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Summary

Tyrosine phosphorylation is an essential step in the transduction of extracellular stimuli into target cells. Here, we describe a method which allows the localization of phosphotyrosine nonradioactively. Solid phase sequencing of phosphotyrosine-containing peptides and subsequent capillary electrophoresis of PTH-phosphotyrosine permits the unambiguous identification of this phosphoamino acid. Less than 1 picomol PTH-phosphotyrosine can be determined without any need for radioactive labelling.

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Authors and Affiliations

  1. Institut für Physiologische Chemie 1, Abteilung Biochemie Supramolekularer Systeme, Protein-Sequenzlabor, MA 2/143, Ruhr-Universität Bochum, D-4630, Bochum, FRG

    H. E. Meyer, E. Hoffmann-Posorke & H. Korte

  2. Dipartimento di Chimica Biologica, Università di Padova, Via Trieste 75, 1-35121, Padova, Italy

    A. Donella-Deana, A. -M. Brunati & L. A. Pinna

  3. Milli Gen/Biosearch, 186 Middelesex Turnpike, 01803, Burlington, MA, USA

    J. Coull

  4. Department of Organic Chemistry, University of Melbourne, 3052, Parkville, Victoria, Australia

    J. Perich, R. M. Valerio & R. B. Johns

Authors
  1. H. E. Meyer
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  2. E. Hoffmann-Posorke
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  3. H. Korte
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  4. A. Donella-Deana
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  5. A. -M. Brunati
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  6. L. A. Pinna
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  7. J. Coull
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  8. J. Perich
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  9. R. M. Valerio
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  10. R. B. Johns
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Meyer, H.E., Hoffmann-Posorke, E., Korte, H. et al. Sequence analysis of phosphotyrosine-containing peptides. Determination of PTH-phosphotyrosine by capillary electrophoresis. Chromatographia 30, 691–695 (1990). https://doi.org/10.1007/BF02269745

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  • DOI: https://doi.org/10.1007/BF02269745

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