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Capillary isotachophoretic analysis of β-lactam antibiotics and their precursors

  • Würzburg Chromatography Colloquium
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Summary

An analytical capillary isotachophoretic method has been developed for the qualitative and quantitative analysis of β-lactam antibiotics, i.e. penicillins and cephalosporins, in chemical and pharmaceutical preparations. This technique also permits the quantitative assay of the β-lactam antibiotics precursors 6-aminopenicillanic acid and 7-aminocephalosporanic acid which are the key materials for the industrial production of semisynthetic penicillins and cephalosporins. Two different electrolyte systems were employed with pH values of the leading electrolytes of 4 and 7. The use of a leading electrolyte with a pH of 7.0 allows the sensitive detection of several β-lactam antibiotics independent of the chemical structure of the sidechain of the penicillin or cephalosporin nucleus. Capillary isotachophoresis is an excellent analytical tool for quality control of β-lactam antibiotics such as penicillins and cephalosporins.

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Authors and Affiliations

  1. Department of Clinical Pharmacology, Medical School Hannover, P.O. Box 610180, D-3000, Hannover 61, FRG

    D. Tsikas

  2. Department of Gastroenterology & Hepatology, Medical School Hannover, P.O. Box 610180, D-3000, Hannover 61, FRG

    A. Hofrichter & G. Brunner

Authors
  1. D. Tsikas
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  2. A. Hofrichter
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  3. G. Brunner
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Tsikas, D., Hofrichter, A. & Brunner, G. Capillary isotachophoretic analysis of β-lactam antibiotics and their precursors. Chromatographia 30, 657–662 (1990). https://doi.org/10.1007/BF02269741

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  • DOI: https://doi.org/10.1007/BF02269741

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