Summary
This paper describes a solventless injection system for preparative supercritical fluid chromatography, which is necessary for injecting large amount of solutes without any prior supercritical fluid extraction procedure. This system eliminates solvent effects on peak broadening. The sample dissolved in a liquid is loaded into a precolumn. A helium flow through the precolumn removes the solvent from the sample and the deposited material remaining is then solvated by the supercritical mobile phase before being injected into the column. In this manner, the band broadening and poor peak shape due to column overload may be studied.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
N. E. Hoffman, S. L. Pan, A. M. Rustum, J. Chromatogr.465, 189 (1989).
A. Farbrot Buskhe, B. E. Berg, O. Gyllenhaal, T. Greibrokk, HRC & CC11, 16 (1988).
B. E. Berg, T. Greibrokk, HRC12, 322 (1989).
T. A. Dean, C. F. Poole, 40th Pittsburg Conference on Analytical Chemistry and Applied Spectroscopy, Atlanta, GA, paper No. 1142 (1989).
M. L. Lee, B. Xu, E. C. Huang, N. M. Djorjevic, H.-C. Chang, K. E. Markides, J. Microcol. Sepn.1, 7 (1989).
T. A. Dean, C. F. Poole, HRC12, 773 (1989).
K. Cross, J. M. Levy, A. C. Russelli, 41th Pittsburg Conference on Analytical Chemistry and Applied Spectroscopy, New York, NY, paper No. 136 (1990).
B. Coq, C. Gonnet, J. L. Rocca, J. Chromatogr.106, 249 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cretier, G., Majdalani, R. & Rocca, J.L. Solventless injection for preparative supercritical fluid chromatography. Chromatographia 30, 645–650 (1990). https://doi.org/10.1007/BF02269739
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02269739