Chromatographia

, Volume 35, Issue 3–4, pp 189–192 | Cite as

An improved HPLC system for the analysis of photosynthetic pigments

  • G. W. Francis
  • B. Huseby
  • Ø. M. Andersen
Originals

Summary

A high performance liquid chromatography system for the analysis of photosynthetic pigments is presented. The method employs an octadecylsilica stationary phase, a programmed quaternary mobile phase consisting of mixtures of methanol, acetonitrile, water and hexane, and a photodiode array detector. Carotenoids and chlorophylls are rapidly analysed in a single chromatographic separation. Thecis-trans isomers of most carotenoids are separated by this method.

Key Words

Column liquid chromatography Carotenoid pigments Chlorophylls Photosynthestic tissue 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1993

Authors and Affiliations

  • G. W. Francis
    • 1
  • B. Huseby
    • 1
  • Ø. M. Andersen
    • 1
  1. 1.Department of ChemistryUniversity of BergenBergenNorway

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