, Volume 38, Issue 11–12, pp 715–722 | Cite as

Evaluation of mobile phase additives in LC-systems using chemometrics

  • A. -M. Andersson
  • A. Karlsson
  • M. Josefson
  • J. Gottfries


A strategy for general optimization of the mobile phase in LC-systems is described in this study. The influence on column efficiencies and capacity factors of three uncharged modifiers, two charged modifiers, pH and column temperature was evaluated with experimental design and multivariate analyses. A factorial design was used and the experimental results demonstrated that the uncharged modifiers and the pH were important for the capacity factors while column temperature and pH were important for the column efficiency. Two different stationary phase materials were used and compared, one was a traditional silica based material and the other a silica covered with a polymer. Both were reversed phase materials. A partial least squares model obtained from the results from the polymer column, Chromspher B, was used to predict retention data.

Key Words

Reversed phase ion-pair chromatography LC-optimization Chemometrics Prediction of retention times 


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  1. [1]
    G. Schill, H. Ehrsson, J. Vessman, D. Westerlund, Separation Methods for Drugs and Related Organic Compounds. Second Edition, (1983), p. 40–77, Swedish Pharmaceutical Press, Stockholm, Sweden.Google Scholar
  2. [2]
    A. Tilly-Melin, Y. Askemark, K.-G. Wahlund, G. Schill, Anal. Chem.51, 976 (1979).CrossRefGoogle Scholar
  3. [3]
    P. J. Schoenmakers, S. V. Molle, C. M. G. Hayes, L. G. M. Uunk, Anal. Chim. Acta250, 1 (1991).CrossRefGoogle Scholar
  4. [4]
    M. Salo, H. Vuorela, J. Halmekoski, J. Chromatogr.592, 127 (1992).CrossRefGoogle Scholar
  5. [5]
    G. E. P. Box, W. G. Hunter, J. S. Hunter, Statistics for Experimenters. (1978), John Wiley & Sons, New York, USA.Google Scholar
  6. [6]
    A. Höskuldsson, J. Chemometrics2, 211 (1988).CrossRefGoogle Scholar
  7. [7]
    M. H. Johnson, C. J. Nachtsheim, Technometrics25, 271 (1983).Google Scholar
  8. [8]
    J. W. Weyland, C. H. P. Bruins, D. A. Doornbos, J Chromatogr. Sci.22, 31 (1984).Google Scholar
  9. [9]
    H. Martens, T. Naes, Multivariate Calibration. (1989), John Wiley & Sons, Chicester, England.Google Scholar
  10. [10]
    S. Wold, Technometrics20, 397 (1978).Google Scholar
  11. [11]
    W. Melander, D. E. Campell, C. Horváth, J. Chromatogr.158, 215 (1978).Google Scholar
  12. [12]
    N. Kettaneh-Wold, Chemometrics and Intelligent Laboratory Systems14, 57 (1992).CrossRefGoogle Scholar
  13. [13]
    J. H. Knox, G. Vasvari, J. Chromatogr.83, 181 (1973).CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • A. -M. Andersson
    • 1
  • A. Karlsson
    • 1
  • M. Josefson
    • 1
  • J. Gottfries
    • 1
  1. 1.Astra Hässle ABMölndalSweden

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