Chromatographia

, Volume 6, Issue 4, pp 187–189 | Cite as

Adsorption and chromatography of proteins on chemically modified macroporous silica-aminosilochrom

  • Yu. A. Eltekov
  • A. V. Kiselev
  • T. D. Khokhlova
  • Yu. S. Nikitin
Originals

Summary

Adsorption and chromatography of several proteins on macroporous silica — Silochrom was investigated. Chemical modification of the Silochrom surface by means of γ-aminopropyltriethoxysilane eliminates irreversible protein adsorption. The effect of salt concentration and pH of eluent on protein elution volumes from “aminosilochrom” columns is discussed. It is possible to use the aminosilochrom for chromatographic separation of proteins.

Keywords

Chromatography Analytical Chemistry Organic Chemistry Salt Concentration Chemical Modification 

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Copyright information

© Friedr. Vieweg & Sohn GmbH 1973

Authors and Affiliations

  • Yu. A. Eltekov
    • 1
    • 2
  • A. V. Kiselev
    • 1
    • 2
  • T. D. Khokhlova
    • 1
    • 2
  • Yu. S. Nikitin
    • 1
    • 2
  1. 1.Laboratory of Adsorption and Gas Chromatography, Department of ChemistryThe M. V. Lomonosov State University of MoscowMoscowU.S.S.R.
  2. 2.Laboratory of Surface Chemistry, Institute of Physical ChemistryU.S.S.R. Academy of SciencesMoscowU.S.S.R.

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