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The use of microparticulate guard columns in reverse-phase high-performance liquid chromatography

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Summary

The use of mciroparticulate guard columns in conjunction with high efficiency reverse-phase columns has been evaluated in terms of system efficiency and peak symmetry using three standard test mixtures. The effects of linking tube dimensions, particle size of guard column packing and the use of low or zero dead volume couplings have been investigated. Both valve and stopped-flow injection techniques have been used. Recommendations are given for the most efficient use of guard columns.

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References

  1. Chrompack News no. 31 (1981).

  2. Supelco Bull. 781 (1979).

  3. Whatman Bull. 112 (1975).

  4. F. M. Rabel, J. Chromatogr. Sci.18, 394 (1980).

    Google Scholar 

  5. J. J. Kirkland, W. W. Yau, H. J. Stoklosa, C. H. Dilks, J. Chromatogr. Sci.15, 303 (1977).

    PubMed  Google Scholar 

  6. A. Schmid, Chromatographia12, 825 (1979).

    Article  Google Scholar 

  7. N. K. Vadukul, C. R. Loscombe, Chromatographia14, 465 (1981).

    Article  Google Scholar 

  8. J. H. Knox (Ed.), High-Performance Liquid Chromatography, Edinburgh University Press, 1978; p. 83.

  9. R. J. Hamilton, P. A. Sewell, Introduction to High Performance Liquid Chromatography, Chapman and Hall, London, 1977; p. 83.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of the Government Chemist, Cornwall House, Stamford Street, SE1 9NQ, London, UK

    G. D. Reed & C. R. Loscombe

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  1. G. D. Reed
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  2. C. R. Loscombe
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Reed, G.D., Loscombe, C.R. The use of microparticulate guard columns in reverse-phase high-performance liquid chromatography. Chromatographia 15, 15–17 (1982). https://doi.org/10.1007/BF02269033

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  • DOI: https://doi.org/10.1007/BF02269033

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