Summary
A high pressure liquid chromatographic method of fatty acid analysis has been developed using the fluorescent label, 4-bromomethyl-7-methoxycoumarin. Two simplified procedures for derivatization using sealed reaction vessels allow derivatization of volumes of 200 to 500 mm3 and 2 to 5 mm3 respectively.
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Zelenski, S.G., Huber, J.W. Application of 4-methyl-7-methoxycoumarin derivatives to the high pressure liquid chromatographic analysis of fatty acids. Chromatographia 11, 645–646 (1978). https://doi.org/10.1007/BF02268998
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DOI: https://doi.org/10.1007/BF02268998