Summary
The present study shows that liquid chromatography, especially as high performance size exclusion chromatography, is a useful tool to characterize newly developed highly permeable membranes, which are used for continuous arteriovenous hemofiltration (CAVH) of patients with renal failure. The molecular weight distribution in the ultrafiltrates of three hemofilters are compared using three different chromatographic systems. Especially in the region of the so-called “higher” molecular weight uremic toxins (MW above 2000 Dalton), the elution patterns of the filtrates show typical membrane-dependent differences. As we have shown earlier, the elimination of these higher molecular weight fractions might be of clinical relevance, as they contain inhibitors of DNA-synthesis in vitro.
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Supported by Deutsche Forschungsgemeinschaft (SFB 109/F5)
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Brunner, H., Schäfer, U., Mann, H. et al. Molecular weight distribution in the ultrafiltrates of different filters used for continuous arteriovenous hemofiltration. Chromatographia 22, 399–403 (1986). https://doi.org/10.1007/BF02268798
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DOI: https://doi.org/10.1007/BF02268798